EIF4B monoclonal antibody, clone 1F5
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of (1) A549 cell, (2) A431 cell, (3) HepG2 cell, (4) HEK293 cell, (5) HeLa cell, (6) Jurkat cell, (7) K562 cell, (8) NIH/3T3 cell, (9) MCF-7 cell with EIF4B monoclonal antibody.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of paraffin-embedded colon cancer tissue with EIF4B monoclonal antibody.
Immunocytochemistry
Immunocytochemical staining of NIH/3T3 cells with EIF4B monoclonal antibody (green). DRAQ5 fluorescent DNA dye (blue). Actin filaments labeled with Alexa Fluor-555 phalloidin (red).
Enzyme-linked Immunoabsorbent Assay
ELISA analysis of EIF4B monoclonal antibody, clone 1F5.
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Specification
Product Description
Mouse monoclonal antibody raised against recombinant human EIF4B.
Immunogen
Recombinant protein corresponding to human EIF4B from E. coli.
Host
Mouse
Theoretical MW (kDa)
80
Reactivity
Human, Mouse
Form
Liquid
Isotype
IgG2b
Recommend Usage
ELISA (1:10000)
Flow Cytometry (1:200-1:400)
Immunocytochemistry (1:200-1:1000)
Immunohistochemistry (1:200-1:1000)
Western Blot (1:500-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.05% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of (1) A549 cell, (2) A431 cell, (3) HepG2 cell, (4) HEK293 cell, (5) HeLa cell, (6) Jurkat cell, (7) K562 cell, (8) NIH/3T3 cell, (9) MCF-7 cell with EIF4B monoclonal antibody.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of paraffin-embedded colon cancer tissue with EIF4B monoclonal antibody.Immunocytochemistry
Immunocytochemical staining of NIH/3T3 cells with EIF4B monoclonal antibody (green). DRAQ5 fluorescent DNA dye (blue). Actin filaments labeled with Alexa Fluor-555 phalloidin (red).Enzyme-linked Immunoabsorbent Assay
ELISA analysis of EIF4B monoclonal antibody, clone 1F5. -
Gene Info — EIF4B
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Interactome
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Pathway
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