RAP1A monoclonal antibody, clone 5F8G2
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More Files
- More Functions
Western Blot (Transfected lysate)
Western blot analysis of (1) HEK293 cells, (2) RAP1A-hIgGFc transfected HEK293 cell lysate with RAP1A monoclonal antibody.
Enzyme-linked Immunoabsorbent Assay
ELISA analysis of RAP1A monoclonal antibody, clone 5F8G2.
Flow Cytometry
Flow cytometric analysis of HeLa cells with RAP1A monoclonal antibody (green) and negative control (red).
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Specification
Product Description
Mouse monoclonal antibody raised against recombinant human RAP1A.
Immunogen
Recombinant protein corresponding to human RAP1A from E. coli.
Host
Mouse
Theoretical MW (kDa)
43.2
Reactivity
Human
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Flow Cytometry (1:200-1:400)
Immunocytochemistry
Immunohistochemistry
Western Blot (1:500-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.05% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western blot analysis of (1) HEK293 cells, (2) RAP1A-hIgGFc transfected HEK293 cell lysate with RAP1A monoclonal antibody.Enzyme-linked Immunoabsorbent Assay
ELISA analysis of RAP1A monoclonal antibody, clone 5F8G2.Flow Cytometry
Flow cytometric analysis of HeLa cells with RAP1A monoclonal antibody (green) and negative control (red). -
Gene Info — RAP1A
Entrez GeneID
5906Gene Name
RAP1A
Gene Alias
KREV-1, KREV1, RAP1, SMGP21
Gene Description
RAP1A, member of RAS oncogene family
Omim ID
179520Gene Ontology
HyperlinkGene Summary
The product of this gene belongs to the family of RAS-related proteins. These proteins share approximately 50% amino acid identity with the classical RAS proteins and have numerous structural features in common. The most striking difference between RAP proteins and RAS proteins resides in their 61st amino acid: glutamine in RAS is replaced by threonine in RAP proteins. The product of this gene counteracts the mitogenic function of RAS because it can interact with RAS GAPs and RAF in a competitive manner. Two transcript variants encoding the same protein have been identified for this gene. [provided by RefSeq
Other Designations
OTTHUMP00000013740|OTTHUMP00000013741|OTTHUMP00000059089|RAS-related protein RAP1A|Ras-related protein Krev-1|Ras-related protein Rap-1A
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Interactome
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Pathway
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Disease
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