HMGB1 monoclonal antibody, clone J2E1
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of Lane 1: Jurkat cell lysate, Lane 2: K562 cell lysate, Lane 3: MCF7 cell lysate.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemistry of human colon cancer tissue were incubated with HMGB1 monoclonal antibody, clone J2E1 (Cat # MAB1771) (1:200) for 2 hours at room temperature. Antigen retrieval was performed in 0.1 M sodium citrate buffer and detected using Diaminobenzidine (DAB).
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Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant HMGB1.
Immunogen
Recombinant protein corresponding to amino acids 1-215 of human HMGB1.
Host
Mouse
Reactivity
Human
Form
Liquid
Purification
Protein G purification
Isotype
IgG2b, kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Recommend Usage
ELISA
Immunohistochemistry (1:200)
Western Blot
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.4 (10% glycerol, 0.02% sodium azide).
Storage Instruction
Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of Lane 1: Jurkat cell lysate, Lane 2: K562 cell lysate, Lane 3: MCF7 cell lysate.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemistry of human colon cancer tissue were incubated with HMGB1 monoclonal antibody, clone J2E1 (Cat # MAB1771) (1:200) for 2 hours at room temperature. Antigen retrieval was performed in 0.1 M sodium citrate buffer and detected using Diaminobenzidine (DAB).Enzyme-linked Immunoabsorbent Assay
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Gene Info — HMGB1
Entrez GeneID
3146Protein Accession#
P09429Gene Name
HMGB1
Gene Alias
DKFZp686A04236, HMG1, HMG3, SBP-1
Gene Description
high-mobility group box 1
Omim ID
163905Gene Ontology
HyperlinkOther Designations
Amphoterin|OTTHUMP00000018199|OTTHUMP00000190860|Sulfoglucuronyl carbohydrate binding protein|high mobility group box 1|high mobility group protein 1|high-mobility group (nonhistone chromosomal) protein 1
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Interactome
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Pathway
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Disease
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Publication Reference
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Extracellular HMGB1, a signal of tissue damage, induces mesoangioblast migration and proliferation.
Palumbo R, Sampaolesi M, De Marchis F, Tonlorenzi R, Colombetti S, Mondino A, Cossu G, Bianchi ME.
The Journal of Cell Biology 2004 Feb; 164(3):441.
Application：IF, IHC-Fr, WB, Mouse, D16 cells, Mouse tibialis anterior muscle sections.
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HMGB1 as a DNA-binding cytokine.
Andersson U, Erlandsson-Harris H, Yang H, Tracey KJ.
Journal of Leukocyte Biology 2002 Dec; 72(6):1084.
Application：IHC, Rat, Synovial.
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Extracellular HMGB1, a signal of tissue damage, induces mesoangioblast migration and proliferation.
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