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Last updated: 2017/2/19
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HMGB1 monoclonal antibody, clone J2E1

  • Catalog # : MAB1771
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  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against partial recombinant HMGB1.
  • Immunogen:
  • Recombinant protein corresponding to amino acids 1-215 of human HMGB1.
  • Host:
  • Mouse
  • Reactivity:
  • Human
  • Form:
  • Liquid
  • Isotype:
  • IgG2b, kappa
  • Quality Control Testing:
  • Antibody Reactive Against Recombinant Protein.
  • Recommend Usage:
  • Western Blot (1:500-1:2000)
    Immunohistochemistry (1:100-1:300)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS, pH 7.4 (0.09% sodium azide)
  • Storage Instruction:
  • Store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Cell lysates of Jurkat (lane 1) and HeLa (lane 2) (30 ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with HMGB1 monoclonal antibody, clone J2E1 (Cat # MAB1771) (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Paraffin embedded sections of human colon cancer tissue were incubated with HMGB1 monoclonal antibody, clone J2E1 (Cat # MAB1771) (1:200) for 2 hours at room temperature. Antigen retrieval was performed in 0.1 M sodium citrate buffer and detected using Diaminobenzidine (DAB).
  • ELISA
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • ELISA
  • Gene Information
  • Entrez GeneID:
  • 3146
  • Gene Name:
  • HMGB1
  • Gene Alias:
  • DKFZp686A04236,HMG1,HMG3,SBP-1
  • Gene Description:
  • high-mobility group box 1
  • Other Designations:
  • Amphoterin,OTTHUMP00000018199,OTTHUMP00000190860,Sulfoglucuronyl carbohydrate binding protein,high mobility group box 1,high mobility group protein 1,high-mobility group (nonhistone chromosomal) protein 1
  • Gene Pathway
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