STAT6 monoclonal antibody, clone 7D3
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of (1) HEK293 cell, (2) NIH/3T3 cell, (3) MCF-7 cell, (4) Raw246.7 cell, (5) PC-12 cell with STAT6 monoclonal antibody.
Immunocytochemistry
Immunocytochemical staining of HeLa cells with STAT6 monoclonal antibody (green). DRAQ5 fluorescent DNA dye (blue). Actin filaments labeled with Alexa Fluor-555 phalloidin (red).
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Specification
Product Description
Mouse monoclonal antibody raised against recombinant human STAT6.
Immunogen
Recombinant protein corresponding to human STAT6 from E. coli.
Host
Mouse
Theoretical MW (kDa)
94
Reactivity
Human, Monkey, Mouse, Rat
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Flow Cytometry
Immunocytochemistry (1:200-1:1000)
Immunohistochemistry
Western Blot (1:500-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In ascites (0.03% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of (1) HEK293 cell, (2) NIH/3T3 cell, (3) MCF-7 cell, (4) Raw246.7 cell, (5) PC-12 cell with STAT6 monoclonal antibody.Immunocytochemistry
Immunocytochemical staining of HeLa cells with STAT6 monoclonal antibody (green). DRAQ5 fluorescent DNA dye (blue). Actin filaments labeled with Alexa Fluor-555 phalloidin (red). -
Gene Info — STAT6
Entrez GeneID
6778Gene Name
STAT6
Gene Alias
D12S1644, IL-4-STAT, STAT6B, STAT6C
Gene Description
signal transducer and activator of transcription 6, interleukin-4 induced
Omim ID
601512Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of the STAT family of transcription factors. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein plays a central role in exerting IL4 mediated biological responses. It is found to induce the expression of BCL2L1/BCL-X(L), which is responsible for the anti-apoptotic activity of IL4. Knockout studies in mice suggested the roles of this gene in differentiation of T helper 2 (Th2) cells, expression of cell surface markers, and class switch of immunoglobulins. [provided by RefSeq
Other Designations
STAT, interleukin4-induced|signal transducer and activator of transcription 6|transcription factor IL-4 STAT
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Interactome
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Pathway
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Disease
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