Cell lysates of LNCap (lane 1), Hep3B (lane 2) (30 ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with ACOT11 monoclonal antibody, clone J4B2 (Cat # MAB1770) (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Paraffin embedded sections of human colon cancer tissue were incubated with ACOT11 monoclonal antibody, clone J4B2 (Cat # MAB1770) (1:100) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB).
This gene encodes a protein with acyl-CoA thioesterase activity towards medium (C12) and long-chain (C18) fatty acyl-CoA substrates. Expression of a similar murine protein in brown adipose tissue is induced by cold exposure and repressed by warmth. Expression of the mouse protein has been associated with obesity, with higher expression found in obesity-resistant mice compared with obesity-prone mice. Alternative splicing results in two transcript variants encoding different isoforms. [provided by RefSeq
OTTHUMP00000011526,OTTHUMP00000011527,OTTHUMP00000046722,START domain containing 14,StAR-related lipid transfer (START) domain containing 14,brown fat inducible thioesterase,thioesterase superfamily member 1,thioesterase, adipose associated