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Last updated: 2016/12/4
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ACOT11 monoclonal antibody, clone J4B2

  • Catalog # : MAB1770
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  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against partial recombinant ACOT11.
  • Immunogen:
  • Recombinant protein corresponding to amino acids 19-250 of human ACOT11.
  • Host:
  • Mouse
  • Reactivity:
  • Human
  • Form:
  • Liquid
  • Isotype:
  • IgG2b, kappa
  • Quality Control Testing:
  • Antibody Reactive Against Recombinant Protein.
  • Recommend Usage:
  • Western Blot (1:500-1:2000)
    Immunohistochemistry (1:100-1:200)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS, pH 7.4 (0.09% sodium azide)
  • Storage Instruction:
  • Store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Cell lysates of LNCap (lane 1), Hep3B (lane 2) (30 ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with ACOT11 monoclonal antibody, clone J4B2 (Cat # MAB1770) (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Paraffin embedded sections of human colon cancer tissue were incubated with ACOT11 monoclonal antibody, clone J4B2 (Cat # MAB1770) (1:100) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB).
  • ELISA
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • ELISA
  • Gene Information
  • Gene Name:
  • ACOT11
  • Gene Alias:
  • BFIT,BFIT1,BFIT2,KIAA0707,STARD14,THEA,THEM1
  • Gene Description:
  • acyl-CoA thioesterase 11
  • Gene Summary:
  • This gene encodes a protein with acyl-CoA thioesterase activity towards medium (C12) and long-chain (C18) fatty acyl-CoA substrates. Expression of a similar murine protein in brown adipose tissue is induced by cold exposure and repressed by warmth. Expression of the mouse protein has been associated with obesity, with higher expression found in obesity-resistant mice compared with obesity-prone mice. Alternative splicing results in two transcript variants encoding different isoforms. [provided by RefSeq
  • Other Designations:
  • OTTHUMP00000011526,OTTHUMP00000011527,OTTHUMP00000046722,START domain containing 14,StAR-related lipid transfer (START) domain containing 14,brown fat inducible thioesterase,thioesterase superfamily member 1,thioesterase, adipose associated
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