CDC25C monoclonal antibody, clone 1F12
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Specification
Product Description
Mouse monoclonal antibody raised against recombinant human CDC25C.
Immunogen
Recombinant protein corresponding to human CDC25C from E. coli.
Host
Mouse
Theoretical MW (kDa)
58
Reactivity
Human
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Flow Cytometry
Immunocytochemistry
Immunohistochemistry (1:200-1:1000)
Western Blot (1:500-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In ascites (0.03% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of (1) HeLa cell, (2) K562 cell, (3) PC-3 cell, (4) HEK293 cell, (5) Raw264.7 cell with CDC25C monoclonal antibody.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of paraffin-embedded human testis tissue with CDC25C monoclonal antibody. -
Gene Info — CDC25C
Entrez GeneID
995Gene Name
CDC25C
Gene Alias
CDC25
Gene Description
cell division cycle 25 homolog C (S. pombe)
Omim ID
157680Gene Ontology
HyperlinkGene Summary
This gene is highly conserved during evolution and it plays a key role in the regulation of cell division. The encoded protein is a tyrosine phosphatase and belongs to the Cdc25 phosphatase family. It directs dephosphorylation of cyclin B-bound CDC2 and triggers entry into mitosis. It is also thought to suppress p53-induced growth arrest. Multiple alternatively spliced transcript variants of this gene have been described, however, the full-length nature of many of them is not known. [provided by RefSeq
Other Designations
cell division cycle 25C|cell division cycle 25C protein|dual specificity phosphatase CDC25C|m-phase inducer phosphatase 3|mitosis inducer CDC25|phosphotyrosine phosphatase
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Interactome
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Pathway
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Disease
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