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Last updated: 2017/4/23
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EPHA2 monoclonal antibody, clone 3D7

  • Catalog # : MAB1769
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  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against partial recombinant EPHA2.
  • Immunogen:
  • Recombinant protein corresponding to amino acids 559-976 of human EPHA2.
  • Host:
  • Mouse
  • Reactivity:
  • Human, Mouse
  • Form:
  • Liquid
  • Isotype:
  • IgG2b, kappa
  • Quality Control Testing:
  • Antibody Reactive Against Recombinant Protein.
  • Recommend Usage:
  • Western Blot (1:500-1:2000)
    Immunohistochemistry (1:100-1:200)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS, pH 7.4 (0.09% sodium azide)
  • Storage Instruction:
  • Store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot (Tissue lysate)
  • Western Blot (Tissue lysate)
  • The extracts of mouse brain (50 ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with EPHA2 monoclonal antibody, clone 3D7 (Cat # MAB1769) (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Paraffin embedded sections of human colon (A) and gastric cancer tissue (B) were incubated with EPHA2 monoclonal antibody, clone 3D7 (Cat # MAB1769) (1:100) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB).
  • ELISA
  • Application Image
  • Western Blot (Tissue lysate)
  • Western Blot (Tissue lysate)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • ELISA
  • Gene Information
  • Entrez GeneID:
  • 1969
  • Gene Name:
  • EPHA2
  • Gene Alias:
  • ECK
  • Gene Description:
  • EPH receptor A2
  • Gene Summary:
  • This gene belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family. EPH and EPH-related receptors have been implicated in mediating developmental events, particularly in the nervous system. Receptors in the EPH subfamily typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. The ephrin receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. This gene encodes a protein that binds ephrin-A ligands. [provided by RefSeq
  • Other Designations:
  • ephrin receptor EphA2,epithelial cell receptor protein tyrosine kinase,protein tyrosine kinase,receptor protein tyrosine kinase regulated by p53 and E2F-1,soluble EPHA2 variant 1
  • Gene Pathway
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