CD3G monoclonal antibody, clone 5B7F9
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More Files
- More Functions
Western Blot (Transfected lysate)
Western blot analysis of (1) HEK293 cells, (2) CD3G-hIgGFc transfected HEK293 cell lysate.
Enzyme-linked Immunoabsorbent Assay
ELISA analysis of CD3G monoclonal antibody, clone 5B7F9.
Flow Cytometry
Flow cytometric analysis of Raji cells with CD3G monoclonal antibody (green) and negative control (red).
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Specification
Product Description
Mouse monoclonal antibody raised against recombinant human CD3G.
Immunogen
Recombinant protein corresponding to amino acid 23-116 of human CD3G from E. coli.
Host
Mouse
Theoretical MW (kDa)
20.5
Reactivity
Human
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Western Blot (1:500-1:2000)
Immunocytochemistry
Flow Cytometry (1:200-1:400)
Immunohistochemistry
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.05% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western blot analysis of (1) HEK293 cells, (2) CD3G-hIgGFc transfected HEK293 cell lysate.Enzyme-linked Immunoabsorbent Assay
ELISA analysis of CD3G monoclonal antibody, clone 5B7F9.Flow Cytometry
Flow cytometric analysis of Raji cells with CD3G monoclonal antibody (green) and negative control (red). -
Gene Info — CD3G
Entrez GeneID
917Gene Name
CD3G
Gene Alias
CD3-GAMMA, FLJ17620, FLJ17664, FLJ79544, FLJ94613, MGC138597, T3G
Gene Description
CD3g molecule, gamma (CD3-TCR complex)
Omim ID
186740Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is the CD3-gamma polypeptide, which together with CD3-epsilon, -delta and -zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T-cell receptor-CD3 complex. This complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. The genes encoding the epsilon, gamma and delta polypeptides are located in the same cluster on chromosome 11. Defects in this gene are associated with T cell immunodeficiency. [provided by RefSeq
Other Designations
CD3G antigen, gamma polypeptide|CD3g antigen, gamma polypeptide (TiT3 complex)|T-cell antigen receptor complex, gamma subunit of T3|T-cell receptor T3 gamma chain|T-cell surface glycoprotein CD3 gamma chain
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Interactome
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Pathway
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Disease
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