FOLR1 monoclonal antibody, clone 2G5C12
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Specification
Product Description
Mouse monoclonal antibody raised against recombinant human FOLR1.
Immunogen
Recombinant protein corresponding to amino acid 41-227 of human FOLR1 from E. coli.
Host
Mouse
Theoretical MW (kDa)
29.8
Reactivity
Human
Form
Liquid
Isotype
IgG2a
Recommend Usage
ELISA (1:10000)
Western Blot (1:500-1:2000)
Immunocytochemistry (1:100-1:500)
Flow Cytometry (1:200-1:400)
Immunohistochemistry (1:200-1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.05% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western blot analysis of (1) HEK293 cells, (2) FOLR1-hIgGFc transfected HEK293 cell lysate.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of paraffin-embedded cervical cancer tissue with FOLR1 monoclonal antibody.Immunocytochemistry
Immunocytochemical staining of HeLa cells with FOLR1 monoclonal antibody (green). DRAQ5 fluorescent DNA dye (blue). Actin filaments labeled with Alexa Fluor-555 phalloidin (red).Enzyme-linked Immunoabsorbent Assay
ELISA analysis of FOLR1 monoclonal antibody, clone 2G5C12.Flow Cytometry
Flow cytometric analysis of Hela cells with FOLR1 monoclonal antibody (green) and negative control (red). -
Gene Info — FOLR1
Entrez GeneID
2348Gene Name
FOLR1
Gene Alias
FBP, FOLR, FR-alpha, MOv18
Gene Description
folate receptor 1 (adult)
Omim ID
136430Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of the folate receptor (FOLR) family. Members of this gene family have a high affinity for folic acid and for several reduced folic acid derivatives, and mediate delivery of 5-methyltetrahydrofolate to the interior of cells. This gene is composed of 7 exons; exons 1 through 4 encode the 5' UTR and exons 4 through 7 encode the open reading frame. Due to the presence of 2 promoters, multiple transcription start sites, and alternative splicing of exons, several transcript variants are derived from this gene. These variants differ in the lengths of 5' and 3' UTR, but they encode an identical amino acid sequence. [provided by RefSeq
Other Designations
folate binding protein|folate receptor 1
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Interactome
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Disease
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