P2RY8 monoclonal antibody, clone 1G5A2
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More Files
- More Functions
Western Blot (Transfected lysate)
Western blot analysis of (1) HEK293 cells, (2) P2RY8-hIgGFc transfected HEK293 cell lysate.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of paraffin-embedded cervical cancer tissue with P2RY8 monoclonal antibody.
Immunocytochemistry
Immunocytochemical staining of HeLa cells with P2RY8 monoclonal antibody (green). DRAQ5 fluorescent DNA dye (blue). Actin filaments labeled with Alexa Fluor-555 phalloidin (red).
Enzyme-linked Immunoabsorbent Assay
ELISA analysis of P2RY8 monoclonal antibody, clone 1G5A2.
Flow Cytometry
Flow cytometric analysis of Hela cells with P2RY8 monoclonal antibody (green) and negative control (red).
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Specification
Product Description
Mouse monoclonal antibody raised against recombinant human P2RY8.
Immunogen
Recombinant protein corresponding to amino acid of human P2RY8 from E. coli.
Host
Mouse
Theoretical MW (kDa)
40.6
Reactivity
Human
Form
Liquid
Isotype
IgG2a
Recommend Usage
ELISA (1:10000)
Western Blot (1:500-1:2000)
Immunocytochemistry (1:100-1:500)
Flow Cytometry (1:200-1:400)
Immunohistochemistry (1:200-1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.05% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western blot analysis of (1) HEK293 cells, (2) P2RY8-hIgGFc transfected HEK293 cell lysate.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of paraffin-embedded cervical cancer tissue with P2RY8 monoclonal antibody.Immunocytochemistry
Immunocytochemical staining of HeLa cells with P2RY8 monoclonal antibody (green). DRAQ5 fluorescent DNA dye (blue). Actin filaments labeled with Alexa Fluor-555 phalloidin (red).Enzyme-linked Immunoabsorbent Assay
ELISA analysis of P2RY8 monoclonal antibody, clone 1G5A2.Flow Cytometry
Flow cytometric analysis of Hela cells with P2RY8 monoclonal antibody (green) and negative control (red). -
Gene Info — P2RY8
Entrez GeneID
286530Gene Name
P2RY8
Gene Alias
MGC50878, P2Y8
Gene Description
purinergic receptor P2Y, G-protein coupled, 8
Omim ID
300525Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene belongs to the family of G-protein coupled receptors, that are preferentially activated by adenosine and uridine nucleotides. This gene is moderately expressed in undifferentiated HL60 cells, and is located on both chromosomes X and Y. [provided by RefSeq
Other Designations
G-protein coupled purinergic receptor P2Y8|OTTHUMP00000022830
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Interactome
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Pathway
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