ATPIF1 monoclonal antibody, clone 1F3B8
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More Files
- More Functions
Western Blot (Transfected lysate)
Western blot analysis of (1) HEK293 cells, (2) ATPIF1-hIgGFc transfected HEK293 cell lysate.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of paraffin-embedded lung cancer tissues with ATPIF1 monoclonal antibody.
Immunocytochemistry
Immunocytochemical staining of HeLa cells with ATPIF1 monoclonal antibody (green). DRAQ5 fluorescent DNA dye (blue). Actin filaments labeled with Alexa Fluor-555 phalloidin (red).
Enzyme-linked Immunoabsorbent Assay
ELISA analysis of ATPIF1 monoclonal antibody, clone 1F3B8.
Flow Cytometry
Flow cytometric analysis of Hela cells with ATPIF1 monoclonal antibody (green) and negative control (red).
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Specification
Product Description
Mouse monoclonal antibody raised against recombinant human ATPIF1.
Immunogen
Recombinant protein corresponding to amino acid 1-106 of human ATPIF1 from E. coli.
Host
Mouse
Theoretical MW (kDa)
12.3
Reactivity
Human
Form
Liquid
Isotype
IgG2b
Recommend Usage
ELISA (1:10000)
Western Blot (1:500-1:2000)
Immunocytochemistry (1:100-1:500)
Flow Cytometry (1:200-1:400)
Immunohistochemistry (1:200-1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.05% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western blot analysis of (1) HEK293 cells, (2) ATPIF1-hIgGFc transfected HEK293 cell lysate.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of paraffin-embedded lung cancer tissues with ATPIF1 monoclonal antibody.Immunocytochemistry
Immunocytochemical staining of HeLa cells with ATPIF1 monoclonal antibody (green). DRAQ5 fluorescent DNA dye (blue). Actin filaments labeled with Alexa Fluor-555 phalloidin (red).Enzyme-linked Immunoabsorbent Assay
ELISA analysis of ATPIF1 monoclonal antibody, clone 1F3B8.Flow Cytometry
Flow cytometric analysis of Hela cells with ATPIF1 monoclonal antibody (green) and negative control (red). -
Gene Info — ATPIF1
Entrez GeneID
93974Gene Name
ATPIF1
Gene Alias
ATPI, ATPIP, IP, MGC1167, MGC8898
Gene Description
ATPase inhibitory factor 1
Gene Ontology
HyperlinkGene Summary
This gene encodes a mitochondrial ATPase inhibitor. Alternative splicing occurs at this locus and three transcript variants encoding distinct isoforms have been identified. [provided by RefSeq
Other Designations
ATP synthase inhibitor protein|ATPase inhibitor protein|OTTHUMP00000003642
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Interactome
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Disease
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