CHRNA7 monoclonal antibody, clone 7F10G1
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More Files
- More Functions
Western Blot (Transfected lysate)
Western blot analysis of (1) HEK293 cells, (2) CHRNA7-hIgGFc transfected HEK293 cell lysate.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of paraffin-embedded bladder cancer tissues with CHRNA7 monoclonal antibody.
Enzyme-linked Immunoabsorbent Assay
ELISA analysis of CHRNA7 monoclonal antibody, clone 7F10G1.
Flow Cytometry
Flow cytometric analysis of SH-SY5Y cells with CHRNA7 monoclonal antibody (green) and negative control (red).
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Specification
Product Description
Mouse monoclonal antibody raised against recombinant human CHRNA7.
Immunogen
Recombinant protein corresponding to amino acid 52-259 of human CHRNA7 from E. coli.
Host
Mouse
Theoretical MW (kDa)
56.4
Reactivity
Human
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Western Blot (1:500-1:2000)
Immunocytochemistry
Flow Cytometry (1:200-1:400)
Immunohistochemistry (1:200-1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.05% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western blot analysis of (1) HEK293 cells, (2) CHRNA7-hIgGFc transfected HEK293 cell lysate.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of paraffin-embedded bladder cancer tissues with CHRNA7 monoclonal antibody.Enzyme-linked Immunoabsorbent Assay
ELISA analysis of CHRNA7 monoclonal antibody, clone 7F10G1.Flow Cytometry
Flow cytometric analysis of SH-SY5Y cells with CHRNA7 monoclonal antibody (green) and negative control (red). -
Gene Info — CHRNA7
Entrez GeneID
1139Gene Name
CHRNA7
Gene Alias
CHRNA7-2, NACHRA7
Gene Description
cholinergic receptor, nicotinic, alpha 7
Omim ID
118511Gene Ontology
HyperlinkGene Summary
The nicotinic acetylcholine receptors (nAChRs) are members of a superfamily of ligand-gated ion channels that mediate fast signal transmission at synapses. The nAChRs are thought to be hetero-pentamers composed of homologous subunits. The proposed structure for each subunit is a conserved N-terminal extracellular domain followed by three conserved transmembrane domains, a variable cytoplasmic loop, a fourth conserved transmembrane domain, and a short C-terminal extracellular region. The protein encoded by this gene forms a homo-oligomeric channel, displays marked permeability to calcium ions and is a major component of brain nicotinic receptors that are blocked by, and highly sensitive to, alpha-bungarotoxin. Once this receptor binds acetylcholine, it undergoes an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane. This gene is located in a region identified as a major susceptibility locus for juvenile myoclonic epilepsy and a chromosomal location involved in the genetic transmission of schizophrenia. An evolutionarily recent partial duplication event in this region results in a hybrid containing sequence from this gene and a novel FAM7A gene. [provided by RefSeq
Other Designations
a7 nicotinic acetylcholine receptor|alpha 7 neuronal nicotinic acetylcholine receptor|alpha-7 nicotinic cholinergic receptor subunit|cholinergic receptor, nicotinic, alpha polypeptide 7|neuronal acetylcholine receptor protein, alpha-7 chain
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Interactome
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Pathway
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Disease
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