GRM3 monoclonal antibody, clone 7A5A6
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More Files
- More Functions
Western Blot (Transfected lysate)
Western blot analysis of (1) HEK293 cells, (2) GRM3-hIgGFc transfected HEK293 cell lysate.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of paraffin-embedded cervical cancer tissue with GRM3 monoclonal antibody.
Immunocytochemistry
Immunocytochemical staining of HeLa cells with GRM3 monoclonal antibody (green). DRAQ5 fluorescent DNA dye (blue). Actin filaments labeled with Alexa Fluor-555 phalloidin (red).
Enzyme-linked Immunoabsorbent Assay
ELISA analysis of GRM3 monoclonal antibody, clone 7A5A6.
Flow Cytometry
Flow cytometric analysis of SH-SY5Y cells with GRM3 monoclonal antibody (green) and negative control (red).
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Specification
Product Description
Mouse monoclonal antibody raised against recombinant human GRM3.
Immunogen
Recombinant protein corresponding to amino acid 433-576 of human GRM3 from E. coli.
Host
Mouse
Theoretical MW (kDa)
99
Reactivity
Human
Form
Liquid
Isotype
IgG2a
Recommend Usage
ELISA (1:10000)
Western Blot (1:500-1:2000)
Immunocytochemistry (1:200-1:1000)
Flow Cytometry (1:200-1:400)
Immunohistochemistry (1:200-1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.05% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western blot analysis of (1) HEK293 cells, (2) GRM3-hIgGFc transfected HEK293 cell lysate.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of paraffin-embedded cervical cancer tissue with GRM3 monoclonal antibody.Immunocytochemistry
Immunocytochemical staining of HeLa cells with GRM3 monoclonal antibody (green). DRAQ5 fluorescent DNA dye (blue). Actin filaments labeled with Alexa Fluor-555 phalloidin (red).Enzyme-linked Immunoabsorbent Assay
ELISA analysis of GRM3 monoclonal antibody, clone 7A5A6.Flow Cytometry
Flow cytometric analysis of SH-SY5Y cells with GRM3 monoclonal antibody (green) and negative control (red). -
Gene Info — GRM3
Entrez GeneID
2913Gene Name
GRM3
Gene Alias
GLUR3, GPRC1C, MGLUR3, mGlu3
Gene Description
glutamate receptor, metabotropic 3
Omim ID
601115Gene Ontology
HyperlinkGene Summary
L-glutamate is the major excitatory neurotransmitter in the central nervous system and activates both ionotropic and metabotropic glutamate receptors. Glutamatergic neurotransmission is involved in most aspects of normal brain function and can be perturbed in many neuropathologic conditions. The metabotropic glutamate receptors are a family of G protein-coupled receptors, that have been divided into 3 groups on the basis of sequence homology, putative signal transduction mechanisms, and pharmacologic properties. Group I includes GRM1 and GRM5 and these receptors have been shown to activate phospholipase C. Group II includes GRM2 and GRM3 while Group III includes GRM4, GRM6, GRM7 and GRM8. Group II and III receptors are linked to the inhibition of the cyclic AMP cascade but differ in their agonist selectivities. [provided by RefSeq
Other Designations
glutamate metabotropic receptor 3
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Interactome
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Pathway
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Disease
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