BTRC monoclonal antibody, clone 4C5D8
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of (1) Ramos cell, (2) MCF-7 cell, (3) K562 cell with BTRC monoclonal antibody.
Western Blot (Transfected lysate)
Western blot analysis of (1) HEK293 cells, (2) BTRC-hIgGFc transfected HEK293 cell lysate.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of paraffin-embedded rectum cancer tissues with BTRC monoclonal antibody.
Enzyme-linked Immunoabsorbent Assay
ELISA analysis of BTRC monoclonal antibody, clone 4C5D8.
Flow Cytometry
Flow cytometric analysis of Hela cells with BTRC monoclonal antibody (green) and negative control (red).
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Specification
Product Description
Mouse monoclonal antibody raised against recombinant human BTRC.
Immunogen
Recombinant protein corresponding to amino acid 24-151 of human BTRC from E. coli.
Host
Mouse
Theoretical MW (kDa)
68.9
Reactivity
Human
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Western Blot (1:500-1:2000)
Immunocytochemistry
Flow Cytometry (1:200-1:400)
Immunohistochemistry (1:200-1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.05% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of (1) Ramos cell, (2) MCF-7 cell, (3) K562 cell with BTRC monoclonal antibody.Western Blot (Transfected lysate)
Western blot analysis of (1) HEK293 cells, (2) BTRC-hIgGFc transfected HEK293 cell lysate.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of paraffin-embedded rectum cancer tissues with BTRC monoclonal antibody.Enzyme-linked Immunoabsorbent Assay
ELISA analysis of BTRC monoclonal antibody, clone 4C5D8.Flow Cytometry
Flow cytometric analysis of Hela cells with BTRC monoclonal antibody (green) and negative control (red). -
Gene Info — BTRC
Entrez GeneID
8945Gene Name
BTRC
Gene Alias
BETA-TRCP, FBW1A, FBXW1, FBXW1A, FWD1, MGC4643, bTrCP, bTrCP1, betaTrCP
Gene Description
beta-transducin repeat containing
Omim ID
603482Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbws class; in addition to an F-box, this protein contains multiple WD-40 repeats. This protein is homologous to Xenopus bTrCP1, yeast Met30, Neurospora Scon2 and Drosophila Slimb proteins. It interacts with HIV-1 Vpu and connects CD4 to the proteolytic machinery. It also associates specifically with phosphorylated IkappaBalpha and beta-catenin destruction motifs, probably functioning in multiple transcriptional programs by activating the NF-kappaB pathway and inhibiting the beta-catenin pathway. [provided by RefSeq
Other Designations
OTTHUMP00000020314|OTTHUMP00000020315|beta-TrCP1|beta-transducin repeat containing protein
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Interactome
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Pathway
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Disease
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