BCL2L10 monoclonal antibody, clone 8A2F9
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More Files
- More Functions
Western Blot (Transfected lysate)
Western blot analysis of (1) HEK293 cells, (2) BCL2L10-hIgGFc transfected HEK293 cell lysate.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of paraffin-embedded lung cancer tissues with BCL2L10 monoclonal antibody.
Immunocytochemistry
Immunocytochemical staining of HeLa cells with BCL2L10 monoclonal antibody (green). DRAQ5 fluorescent DNA dye (blue). Actin filaments labeled with Alexa Fluor-555 phalloidin (red).
Enzyme-linked Immunoabsorbent Assay
ELISA analysis of BCL2L10 monoclonal antibody, clone 8A2F9.
Flow Cytometry
Flow cytometric analysis of Hela cells with BCL2L10 monoclonal antibody (green) and negative control (red).
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Specification
Product Description
Mouse monoclonal antibody raised against recombinant human BCL2L10.
Immunogen
Recombinant protein corresponding to amino acid 31-186 of human BCL2L10 from E. coli.
Host
Mouse
Theoretical MW (kDa)
22
Reactivity
Human
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Western Blot (1:500-1:2000)
Immunocytochemistry (1:200-1:1000)
Flow Cytometry (1:200-1:400)
Immunohistochemistry (1:200-1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.05% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western blot analysis of (1) HEK293 cells, (2) BCL2L10-hIgGFc transfected HEK293 cell lysate.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of paraffin-embedded lung cancer tissues with BCL2L10 monoclonal antibody.Immunocytochemistry
Immunocytochemical staining of HeLa cells with BCL2L10 monoclonal antibody (green). DRAQ5 fluorescent DNA dye (blue). Actin filaments labeled with Alexa Fluor-555 phalloidin (red).Enzyme-linked Immunoabsorbent Assay
ELISA analysis of BCL2L10 monoclonal antibody, clone 8A2F9.Flow Cytometry
Flow cytometric analysis of Hela cells with BCL2L10 monoclonal antibody (green) and negative control (red). -
Gene Info — BCL2L10
Entrez GeneID
10017Gene Name
BCL2L10
Gene Alias
BCL-B, Boo, Diva, MGC129810, MGC129811
Gene Description
BCL2-like 10 (apoptosis facilitator)
Omim ID
606910Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene belongs to the BCL-2 protein family. BCL-2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. The protein encoded by this gene contains conserved BH4, BH1 and BH2 domains. This protein can interact with other members of BCL-2 protein family including BCL2, BCL2L1/BCL-X(L), and BAX. Overexpression of this gene has been shown to suppress cell apoptosis possibly through the prevention of cytochrome C release from the mitochondria, and thus activating caspase-3 activation. The mouse counterpart of this protein is found to interact with Apaf1 and forms a protein complex with Caspase 9, which suggests the involvement of this protein in APAF1 and CASPASE 9 related apoptotic pathway. [provided by RefSeq
Other Designations
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Interactome
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Disease
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