RUNX3 monoclonal antibody, clone 7E2B8
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More Files
- More Functions
Western Blot (Transfected lysate)
Western blot analysis of (1) HEK293 cells, (2) RUNX3-hIgGFc transfected HEK293 cell lysate.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of paraffin-embedded esophageal cancer tissues with RUNX3 monoclonal antibody.
Immunocytochemistry
Immunocytochemical staining of HeLa cells with RUNX3 monoclonal antibody (green). DRAQ5 fluorescent DNA dye (blue). Actin filaments labeled with Alexa Fluor-555 phalloidin (red).
Enzyme-linked Immunoabsorbent Assay
ELISA analysis of RUNX3 monoclonal antibody, clone 7E2B8.
Flow Cytometry
Flow cytometric analysis of Hela cells with RUNX3 monoclonal antibody (green) and negative control (red).
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Specification
Product Description
Mouse monoclonal antibody raised against recombinant human RUNX3.
Immunogen
Recombinant protein corresponding to amino acid 294-429 of human RUNX3 from E. coli.
Host
Mouse
Theoretical MW (kDa)
44.4
Reactivity
Human
Form
Liquid
Isotype
IgG2b
Recommend Usage
ELISA (1:10000)
Western Blot (1:500-1:2000)
Immunocytochemistry (1:50-1:250)
Flow Cytometry (1:200-1:400)
Immunohistochemistry (1:200-1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.05% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western blot analysis of (1) HEK293 cells, (2) RUNX3-hIgGFc transfected HEK293 cell lysate.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of paraffin-embedded esophageal cancer tissues with RUNX3 monoclonal antibody.Immunocytochemistry
Immunocytochemical staining of HeLa cells with RUNX3 monoclonal antibody (green). DRAQ5 fluorescent DNA dye (blue). Actin filaments labeled with Alexa Fluor-555 phalloidin (red).Enzyme-linked Immunoabsorbent Assay
ELISA analysis of RUNX3 monoclonal antibody, clone 7E2B8.Flow Cytometry
Flow cytometric analysis of Hela cells with RUNX3 monoclonal antibody (green) and negative control (red). -
Gene Info — RUNX3
Entrez GeneID
864Gene Name
RUNX3
Gene Alias
AML2, CBFA3, FLJ34510, MGC16070, PEBP2aC
Gene Description
runt-related transcription factor 3
Omim ID
600210Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the runt domain-containing family of transcription factors. A heterodimer of this protein and a beta subunit forms a complex that binds to the core DNA sequence 5'-PYGPYGGT-3' found in a number of enhancers and promoters, and can either activate or suppress transcription. It also interacts with other transcription factors. It functions as a tumor suppressor, and the gene is frequently deleted or transcriptionally silenced in cancer. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
Other Designations
OTTHUMP00000003370|OTTHUMP00000003371|PEA2 alpha C|PEBP2 alpha C|acute myeloid leukemia gene 2|core-binding factor, runt domain, alpha subunit 3|polyomavirus enhancer-binding protein 2 alpha C subunit|transcription factor AML2
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Interactome
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Disease
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