SMARCA1 monoclonal antibody, clone 2H7B9
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Specification
Product Description
Mouse monoclonal antibody raised against recombinant human SMARCA1.
Immunogen
Recombinant protein corresponding to amino acid 933-1070 of human SMARCA1 from E. coli.
Host
Mouse
Theoretical MW (kDa)
122.6
Reactivity
Human, Mouse
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Western Blot (1:500-1:2000)
Immunocytochemistry
Flow Cytometry (1:200-1:400)
Immunohistochemistry (1:200-1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.05% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of (1) PANC-1 cell, (2) HEK293 cell, (3) SW620 cell, (4) HT-29 cell, (5) SH-SY5Y cell, (6) SK-OV-3 cell with SMARCA1 monoclonal antibody.Western Blot (Transfected lysate)
Western blot analysis of (1) HEK293 cells, (2) SMARCA1-hIgGFc transfected HEK293 cell lysate.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of paraffin-embedded esophageal cancer tissues with SMARCA1 monoclonal antibody.Enzyme-linked Immunoabsorbent Assay
ELISA analysis of SMARCA1 monoclonal antibody, clone 2H7B9.Flow Cytometry
Flow cytometric analysis of NIH/3T3 cells with SMARCA1 monoclonal antibody (green) and negative control (red). -
Gene Info — SMARCA1
Entrez GeneID
6594Gene Name
SMARCA1
Gene Alias
DKFZp686D1623, FLJ41547, ISWI, NURF140, SNF2L, SNF2L1, SNF2LB, SWI, SWI2
Gene Description
SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 1
Omim ID
300012Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the SWI/SNF family of proteins. Members of this family have helicase and ATPase activities and are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
Other Designations
OTTHUMP00000023984|OTTHUMP00000062565|SNF2-like 1|SWI/SNF-related matrix-associated actin-dependent regulator of chromatin a1|global transcription activator homologous sequence|sucrose nonfermenting 2-like protein 1
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Interactome
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