ATG7 monoclonal antibody, clone 7D3E5
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More Files
- More Functions
Western Blot (Transfected lysate)
Western blot analysis of (1) HEK293 cells, (2) ATG7-hIgGFc transfected HEK293 cell lysate.
Enzyme-linked Immunoabsorbent Assay
ELISA analysis of ATG7 monoclonal antibody, clone 7D3E5.
Flow Cytometry
Flow cytometric analysis of Hela cells with ATG7 monoclonal antibody (green) and negative control (red).
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Specification
Product Description
Mouse monoclonal antibody raised against recombinant human ATG7.
Immunogen
Recombinant protein corresponding to amino acid 558-703 of human ATG7 from E. coli.
Host
Mouse
Theoretical MW (kDa)
78
Reactivity
Human
Form
Liquid
Isotype
IgG2b
Recommend Usage
ELISA (1:10000)
Western Blot (1:500-1:2000)
Immunocytochemistry
Flow Cytometry (1:200-1:400)
Immunohistochemistry
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.05% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western blot analysis of (1) HEK293 cells, (2) ATG7-hIgGFc transfected HEK293 cell lysate.Enzyme-linked Immunoabsorbent Assay
ELISA analysis of ATG7 monoclonal antibody, clone 7D3E5.Flow Cytometry
Flow cytometric analysis of Hela cells with ATG7 monoclonal antibody (green) and negative control (red). -
Gene Info — ATG7
Entrez GeneID
10533Gene Name
ATG7
Gene Alias
APG7-LIKE, APG7L, DKFZp434N0735, GSA7
Gene Description
ATG7 autophagy related 7 homolog (S. cerevisiae)
Omim ID
608760Gene Ontology
HyperlinkGene Summary
This gene was identified based on homology to Pichia pastoris GSA7 and Saccharomyces cerevisiae APG7. In the yeast, the protein appears to be required for fusion of peroxisomal and vacuolar membranes. The protein shows homology to the ATP-binding and catalytic sites of the E1 ubiquitin activating enzymes. [provided by RefSeq
Other Designations
APG7 autophagy 7-like|ubiquitin activating enzyme E1-like protein
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Interactome
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Pathway
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Disease
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