ANAPC1 monoclonal antibody, clone 7G9C7
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More Files
- More Functions
Western Blot (Transfected lysate)
Western blot analysis of (1) HEK293 cells, (2) ANAPC1-hIgGFc transfected HEK293 cell lysate.
Enzyme-linked Immunoabsorbent Assay
ELISA analysis of ANAPC1 monoclonal antibody, clone 7G9C7.
Flow Cytometry
Flow cytometric analysis of MCF-7 cells with ANAPC1 monoclonal antibody (green) and negative control (red).
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Specification
Product Description
Mouse monoclonal antibody raised against recombinant human ANAPC1.
Immunogen
Recombinant protein corresponding to amino acid 12-155 of human ANAPC1 from E. coli.
Host
Mouse
Theoretical MW (kDa)
216
Reactivity
Human
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Western Blot (1:500-1:2000)
Immunocytochemistry
Flow Cytometry (1:200-1:400)
Immunohistochemistry
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.05% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western blot analysis of (1) HEK293 cells, (2) ANAPC1-hIgGFc transfected HEK293 cell lysate.Enzyme-linked Immunoabsorbent Assay
ELISA analysis of ANAPC1 monoclonal antibody, clone 7G9C7.Flow Cytometry
Flow cytometric analysis of MCF-7 cells with ANAPC1 monoclonal antibody (green) and negative control (red). -
Gene Info — ANAPC1
Entrez GeneID
64682Gene Name
ANAPC1
Gene Alias
APC1, MCPR, TSG24
Gene Description
anaphase promoting complex subunit 1
Omim ID
608473Gene Ontology
HyperlinkGene Summary
ANAPC1 is 1 of at least 10 subunits of the anaphase-promoting complex (APC), which functions at the metaphase-to-anaphase transition of the cell cycle and is regulated by spindle checkpoint proteins. The APC is an E3 ubiquitin ligase that targets cell cycle regulatory proteins for degradation by the proteasome, thereby allowing progression through the cell cycle.[supplied by OMIM
Other Designations
anaphase-promoting complex 1 (meiotic checkpoint regulator)
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Interactome
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Pathway
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