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Last updated: 2021/10/24

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PRKAA2 monoclonal antibody, clone 8E11H5 

  • Catalog # : MAB17500
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  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against recombinant human PRKAA2.
  • Immunogen:
  • Recombinant protein corresponding to amino acid 453-552 of human PRKAA2 from E. coli.
  • Host:
  • Mouse
  • Theoretical MW (kDa):
  • 62.3
  • Reactivity:
  • Human, Monkey
  • Form:
  • Liquid
  • Isotype:
  • IgG1
  • Recommend Usage:
  • ELISA (1:10000)
    Western Blot (1:500-1:2000)
    Immunocytochemistry
    Flow Cytometry (1:200-1:400)
    Immunohistochemistry (1:200-1:1000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS (0.05% sodium azide).
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western blot analysis of (1) HEK293 cell, (2) COS7 cell with PRKAA2 monoclonal antibody.
  • Western Blot (Transfected lysate)
  • Western Blot (Transfected lysate)
  • Western blot analysis of (1) HEK293 cells, (2) PRKAA2-hIgGFc transfected HEK293 cell lysate.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemical staining of paraffin-embedded stomach cancer tissues with PRKAA2 monoclonal antibody.
  • Enzyme-linked Immunoabsorbent Assay
  • Enzyme-linked Immunoabsorbent Assay
  • ELISA analysis of PRKAA2 monoclonal antibody, clone 8E11H5.
  • Flow Cytometry
  • Flow Cytometry
  • Flow cytometric analysis of Jurkat cells with PRKAA2 monoclonal antibody (green) and negative control (red).
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Western Blot (Transfected lysate)
  • Western Blot (Transfected lysate)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • Enzyme-linked Immunoabsorbent Assay
  • Enzyme-linked Immunoabsorbent Assay
  • enlarge
  • Gene Information
  • Entrez GeneID:
  • 5563
  • Gene Name:
  • PRKAA2
  • Gene Alias:
  • AMPK,AMPK2,PRKAA
  • Gene Description:
  • protein kinase, AMP-activated, alpha 2 catalytic subunit
  • Gene Summary:
  • The protein encoded by this gene is a catalytic subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. Studies of the mouse counterpart suggest that this catalytic subunit may control whole-body insulin sensitivity and is necessary for maintaining myocardial energy homeostasis during ischemia. [provided by RefSeq
  • Other Designations:
  • 5'-AMP-activated protein kinase, catalytic alpha-2 chain,AMP-activated protein kinase alpha 2 catalytic subunit,AMPK-alpha-2 chain,OTTHUMP00000009993
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