PRKAA2 monoclonal antibody, clone 8E11H5
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of (1) HEK293 cell, (2) COS7 cell with PRKAA2 monoclonal antibody.
Western Blot (Transfected lysate)
Western blot analysis of (1) HEK293 cells, (2) PRKAA2-hIgGFc transfected HEK293 cell lysate.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of paraffin-embedded stomach cancer tissues with PRKAA2 monoclonal antibody.
Enzyme-linked Immunoabsorbent Assay
ELISA analysis of PRKAA2 monoclonal antibody, clone 8E11H5.
Flow Cytometry
Flow cytometric analysis of Jurkat cells with PRKAA2 monoclonal antibody (green) and negative control (red).
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Specification
Product Description
Mouse monoclonal antibody raised against recombinant human PRKAA2.
Immunogen
Recombinant protein corresponding to amino acid 453-552 of human PRKAA2 from E. coli.
Host
Mouse
Theoretical MW (kDa)
62.3
Reactivity
Human, Monkey
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Western Blot (1:500-1:2000)
Immunocytochemistry
Flow Cytometry (1:200-1:400)
Immunohistochemistry (1:200-1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.05% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of (1) HEK293 cell, (2) COS7 cell with PRKAA2 monoclonal antibody.Western Blot (Transfected lysate)
Western blot analysis of (1) HEK293 cells, (2) PRKAA2-hIgGFc transfected HEK293 cell lysate.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of paraffin-embedded stomach cancer tissues with PRKAA2 monoclonal antibody.Enzyme-linked Immunoabsorbent Assay
ELISA analysis of PRKAA2 monoclonal antibody, clone 8E11H5.Flow Cytometry
Flow cytometric analysis of Jurkat cells with PRKAA2 monoclonal antibody (green) and negative control (red). -
Gene Info — PRKAA2
Entrez GeneID
5563Gene Name
PRKAA2
Gene Alias
AMPK, AMPK2, PRKAA
Gene Description
protein kinase, AMP-activated, alpha 2 catalytic subunit
Omim ID
600497Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a catalytic subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. Studies of the mouse counterpart suggest that this catalytic subunit may control whole-body insulin sensitivity and is necessary for maintaining myocardial energy homeostasis during ischemia. [provided by RefSeq
Other Designations
5'-AMP-activated protein kinase, catalytic alpha-2 chain|AMP-activated protein kinase alpha 2 catalytic subunit|AMPK-alpha-2 chain|OTTHUMP00000009993
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Interactome
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Pathway
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Disease
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