ARD1A monoclonal antibody, clone 3G3B9
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of (1)COS7 cell, (2) HEK293 cell, (3) HL-60 cell, (4) MCF-7 cell, (5) Hela cell, (6) NIH/3T3 cell, (7) C2C12 cell with ARD1A monoclonal antibody.
Western Blot (Transfected lysate)
Western blot analysis of (1) HEK293 cells, (2) ARD1A-hIgGFc transfected HEK293 cell lysate.
Immunocytochemistry
Immunocytochemical staining of HeLa cells with ARD1A monoclonal antibody (green). DRAQ5 fluorescent DNA dye (blue). Actin filaments labeled with Alexa Fluor-555 phalloidin (red).
Enzyme-linked Immunoabsorbent Assay
ELISA analysis of ARD1A monoclonal antibody, clone 3G3B9.
Flow Cytometry
Flow cytometric analysis of SMMC-7721 cells with ARD1A monoclonal antibody (green) and negative control (red).
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Specification
Product Description
Mouse monoclonal antibody raised against recombinant human ARD1A.
Immunogen
Recombinant protein corresponding to amino acid 111-235 of human ARD1A from E. coli.
Host
Mouse
Theoretical MW (kDa)
26.5
Reactivity
Human, Monkey, Mouse
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Western Blot (1:500-1:2000)
Immunocytochemistry (1:200-1:1000)
Flow Cytometry (1:200-1:400)
Immunohistochemistry
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.05% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of (1)COS7 cell, (2) HEK293 cell, (3) HL-60 cell, (4) MCF-7 cell, (5) Hela cell, (6) NIH/3T3 cell, (7) C2C12 cell with ARD1A monoclonal antibody.Western Blot (Transfected lysate)
Western blot analysis of (1) HEK293 cells, (2) ARD1A-hIgGFc transfected HEK293 cell lysate.Immunocytochemistry
Immunocytochemical staining of HeLa cells with ARD1A monoclonal antibody (green). DRAQ5 fluorescent DNA dye (blue). Actin filaments labeled with Alexa Fluor-555 phalloidin (red).Enzyme-linked Immunoabsorbent Assay
ELISA analysis of ARD1A monoclonal antibody, clone 3G3B9.Flow Cytometry
Flow cytometric analysis of SMMC-7721 cells with ARD1A monoclonal antibody (green) and negative control (red). -
Gene Info — ARD1A
Entrez GeneID
8260Gene Name
ARD1A
Gene Alias
ARD1, DXS707, MGC71248, TE2
Gene Description
ARD1 homolog A, N-acetyltransferase (S. cerevisiae)
Omim ID
300013Gene Ontology
HyperlinkGene Summary
N-alpha-acetylation is one of the most common protein modifications that occurs during protein synthesis and involves the transfer of an acetyl group from acetyl-coenzyme A to the protein alpha-amino group. ARD1A, together with NATH (NARG1; MIM 608000), is part of a major N-alpha-acetyltransferase complex responsible for alpha-acetylation of proteins and peptides (Sanchez-Puig and Fersht, 2006 [PubMed 16823041]).[supplied by OMIM
Other Designations
ARD1 homolog, N-acetyltransferase|N-acetyltransferase ARD1|N-acetyltransferase ARD1, human homolog of|OTTHUMP00000026001
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Interactome
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Pathway
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Disease
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