ANAPC10 monoclonal antibody, clone 3E9A9
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of (1) Jurkat cell, (2) MCF-7 cell, (3) SK-Br-3 cell, (4) A431 cell, (5) HEK293 cell, (6) A549 cell, (7) SPC-A-1 cell with ANAPC10 monoclonal antibody.
Western Blot (Transfected lysate)
Western blot analysis of (1) HEK293 cells, (2) ANAPC10-hIgGFc transfected HEK293 cell lysate.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of paraffin-embedded esophageal cancer tissues with ANAPC10 monoclonal antibody.
Immunocytochemistry
Immunocytochemical staining of HeLa cells with ANAPC10 monoclonal antibody (green). DRAQ5 fluorescent DNA dye (blue). Actin filaments labeled with Alexa Fluor-555 phalloidin (red).
Enzyme-linked Immunoabsorbent Assay
ELISA analysis of ANAPC10 monoclonal antibody, clone 3E9A9.
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Specification
Product Description
Mouse monoclonal antibody raised against recombinant human ANAPC10.
Immunogen
Recombinant protein corresponding to amino acid 1-185 of human ANAPC10 from E. coli.
Host
Mouse
Theoretical MW (kDa)
21.3
Reactivity
Human
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Western Blot (1:500-1:2000)
Immunohistochemistry (1:200-1:1000)
Immunocytochemistry (1:200-1:1000)
Flow Cytometry
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.05% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of (1) Jurkat cell, (2) MCF-7 cell, (3) SK-Br-3 cell, (4) A431 cell, (5) HEK293 cell, (6) A549 cell, (7) SPC-A-1 cell with ANAPC10 monoclonal antibody.Western Blot (Transfected lysate)
Western blot analysis of (1) HEK293 cells, (2) ANAPC10-hIgGFc transfected HEK293 cell lysate.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of paraffin-embedded esophageal cancer tissues with ANAPC10 monoclonal antibody.Immunocytochemistry
Immunocytochemical staining of HeLa cells with ANAPC10 monoclonal antibody (green). DRAQ5 fluorescent DNA dye (blue). Actin filaments labeled with Alexa Fluor-555 phalloidin (red).Enzyme-linked Immunoabsorbent Assay
ELISA analysis of ANAPC10 monoclonal antibody, clone 3E9A9. -
Gene Info — ANAPC10
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Interactome
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Pathway
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