4-Hydroxy-2-hexenal monoclonal antibody, clone 6F10 (FITC)
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More Files
- More Functions
Immunocytochemistry
Immunocytochemical staining of HEK293 with 4-Hydroxy-2-hexenal monoclonal antibody, clone 6F10 (FITC) (Cat # MAB17397). (A, B) DAPI (blue) nuclear stain. (C, D) Phalloidin Alex Fluor 633 F-Actin stain. (E, F) 4-Hydroxy-2-hexenal Antibody. (G, H) Composite. (A, C, E, G) Untreated and (B, D, F, H) Cells cultured overnight with 50 uM H2O2.
Flow Cytometry
Flow cytometric analysis of SH-SY5Y with 4-Hydroxy-2-hexenal monoclonal antibody, clone 6F10 (FITC) (Cat # MAB17397).
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Specification
Product Description
Mouse monoclonal antibody raised against synthetic 4-Hydroxy-2-hexenal (4-HHE).
Immunogen
Synthetic 4-Hydroxy-2-hexenal modified Keyhole Limpet Hemocyanin (KLH).
Host
Mouse
Form
Liquid
Conjugation
FITC
Purification
Protein G purification
Isotype
IgG2b
Recommend Usage
ELISA (1:1000)
Flow Cytometry (1:50)
Immunocytochemistry (1:50)
Immunofluorescence (1:50)
Western Blot (1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.4 (50% glycerol, 0.09% sodium azide).
Storage Instruction
Store at -20°C.
Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Immunocytochemistry
Immunocytochemical staining of HEK293 with 4-Hydroxy-2-hexenal monoclonal antibody, clone 6F10 (FITC) (Cat # MAB17397). (A, B) DAPI (blue) nuclear stain. (C, D) Phalloidin Alex Fluor 633 F-Actin stain. (E, F) 4-Hydroxy-2-hexenal Antibody. (G, H) Composite. (A, C, E, G) Untreated and (B, D, F, H) Cells cultured overnight with 50 uM H2O2.Immunofluorescence
Enzyme-linked Immunoabsorbent Assay
Flow Cytometry
Flow cytometric analysis of SH-SY5Y with 4-Hydroxy-2-hexenal monoclonal antibody, clone 6F10 (FITC) (Cat # MAB17397).
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