Macrophage surface antigen monoclonal antibody, clone AM-3K
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Specification
Product Description
Mouse monoclonal antibody raised against macrophage surface antigen.
Immunogen
Native human alveolar macrophages.
Host
Mouse
Reactivity
Human
Form
Liquid
Purification
Protein G affinity chromatography
Isotype
IgG1
Quality Control Testing
Antibody Reactive Against macrophage surface antigen.
Recommend Usage
Immunohistochemistry (10 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.1% proclin, 2.0% Block Ace)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
Immunohistochemistry
Imunohistochemical analysis of human lymph node tissue, using Macrophage surface sntigen monoclonal antibody, clone AM-3K (Cat # MAB1733, 10 ug/mL).Immunohistochemistry
Imunohistochemical analysis of human liver tissue, using Macrophage surface sntigen monoclonal antibody, clone AM-3K (Cat # MAB1733, 10 ug/mL). -
Publication Reference
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High Numbers of CD163-Positive Macrophages in the Fibrotic Region of Exuberant Granulation Tissue in Horses.
Charis Du Cheyne, Ann Martens, Ward De Spiegelaere.
Animals 2021 Sep; 11(9):2728.
Application:IHC-P, Horse, Horse exuberant granulation tissue.
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Quantification of Macrophages and Mycobacterium avium Subsp. paratuberculosis in Bovine Intestinal Tissue During Different Stages of Johne's Disease.
Jenvey CJ, Hostetter JM, Shircliff AL, Bannantine JP, Stabel JR.
Veterinary Pathology 2019 May; 300985819844823.
Application:IF, Bovine, Bovine intestinal tissues.
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Relationship between the pathology of bovine intestinal tissue and current diagnostic tests for Johne's disease.
Jenvey CJ, Hostetter JM, Shircliff AL, Stabel JR.
Veterinary Immunology and Immunopathology 2018 Aug; 202:93.
Application:IF, IHC-Fr, Bovine, Bovine mid-ileal intestinal samples.
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Autofluorescence and Nonspecific Immunofluorescent Labeling in Frozen Bovine Intestinal Tissue Sections: Solutions for Multicolor Immunofluorescence Experiments.
Jenvey CJ, Stabel JR.
The Journal of Histochemistry and Cytochemistry: Official Journal of the Histochemistry Society 2017 Aug; 65(9):531.
Application:IF, IHC-Fr, Bovine, Frozen bovine mid-ileal intestinal tissue sections.
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Distribution of cells immunopositive for AM-3K, a novel monoclonal antibody recognizing human macrophages, in normal and diseased tissues of dogs, cats, horses, cattle, pigs, and rabbits.
Yamate J, Yoshida H, Tsukamoto Y, Ide M, Kuwamura M, Ohashi F, Miyamoto T, Kotani T, Sakuma S, Takeya M.
Veterinary Pathology 2000 Mar; 37(2):168.
Application:ICC, IEM, WB, Dogs, Cats, Horses, Cattle, Pigs, Rabbit, Liver, Kidneys, Lungs, Heart, Thymus, Spleen, Lymph nodes, Pancreas, Small and large intestines, Skin, Brain, Spinal cord, Bone marrow.
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Histochemical and morphological characteristics of canine cardiac mast cells.
Frangogiannis NG, Burns AR, Michael LH, Entman ML.
The Histochemical Journal 1999 Apr; 31(4):221.
Application:IHC-P, Dog, Dog hearts.
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Interspecies reactivities of anti-human macrophage monoclonal antibodies to various animal species.
Zeng L, Takeya M, Ling X, Nagasaki A, Takahashi K.
The Journal of Histochemistry and Cytochemistry 1996 Aug; 44(8):845.
Application:IEM, IHC, WB-Ti, Cat, Dog, Monkey, Mouse, Pig, Rabbit, Rat, Lympho nodes, Macrophages, Spleens, Testes.
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AM-3K, a novel monoclonal antibody specific for tissue macrophages and its application to pathological investigation.
Zeng L, Takeya M, Takahashi K.
The Journal of Pathology 1996 Feb; 178(2):207.
Application:IEM, IHC-Fr, IHC-P, WB, Human, Human alveolar macrophages, Human tissues.
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High Numbers of CD163-Positive Macrophages in the Fibrotic Region of Exuberant Granulation Tissue in Horses.
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