PTPN14 monoclonal antibody, clone 2C12E3
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More Files
- More Functions
Western Blot
Western Blot analysis of human PTPN14 recombinant protein.
Western Blot
Western Blot analysis of (1) HEK293 cells, (2) PTPN14-hIgGFc transfected HEK293 cell lysate.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of (A) rectum cancer tissues and (B) endometrial cancer tissues with DAB staining.
Immunofluorescence
Immunofluorescent staining of HeLa cell line with antibody (green). DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Enzyme-linked Immunoabsorbent Assay
ELISA analysis of PTPN14 monoclonal antibody.
Flow Cytometry
Flow cytometric analysis of HeLa cells using PTPN14 mouse monoclonal antibody (green) and negative control (red).
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Specification
Product Description
Mouse monoclonal antibody raised against recombinant human PTPN14.
Immunogen
Recombinant protein corresponding to amino acids 896-1169 of of human PTPN14 from E. coli.
Host
Mouse
Theoretical MW (kDa)
135.3
Reactivity
Human
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Flow Cytometry (1:200-400)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:200-1000)
Immunofluorescence (1:200-1000)
Western Blot (1:500-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.05% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western Blot analysis of human PTPN14 recombinant protein.Western Blot
Western Blot analysis of (1) HEK293 cells, (2) PTPN14-hIgGFc transfected HEK293 cell lysate.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of (A) rectum cancer tissues and (B) endometrial cancer tissues with DAB staining.Immunofluorescence
Immunofluorescent staining of HeLa cell line with antibody (green). DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.Enzyme-linked Immunoabsorbent Assay
ELISA analysis of PTPN14 monoclonal antibody.Flow Cytometry
Flow cytometric analysis of HeLa cells using PTPN14 mouse monoclonal antibody (green) and negative control (red). -
Gene Info — PTPN14
Entrez GeneID
5784Gene Name
PTPN14
Gene Alias
MGC126803, PEZ, PTP36
Gene Description
protein tyrosine phosphatase, non-receptor type 14
Omim ID
603155Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP contains an N-terminal noncatalytic domain similar to that of band 4.1 superfamily cytoskeleton-associated proteins, which suggested the membrane or cytoskeleton localization of this protein. The specific function of this PTP has not yet been determined. [provided by RefSeq
Other Designations
OTTHUMP00000035094|cytoskeletal-associated protein tyrosine phosphatase
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Interactome
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Disease
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