ARF1 monoclonal antibody, clone 8D8E9
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Specification
Product Description
Mouse monoclonal antibody raised against recombinant human ARF1.
Immunogen
Recombinant protein corresponding to amino acids 76-182 of of human ARF1 from E. coli.
Host
Mouse
Theoretical MW (kDa)
20.7
Reactivity
Human
Form
Liquid
Isotype
IgG2a
Recommend Usage
ELISA (1:10000)
Flow Cytometry (1:200-400)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:200-1000)
Immunofluorescence (1:200-1000)
Western Blot (1:500-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.05% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western Blot analysis of human ARF1 recombinant protein.Western Blot
Western Blot analysis of (1) HEK293 cells, (2) ARF1-hIgGFc transfected HEK293 cell lysate.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of (A) bladder cancer tissues and (B) stomach cancer tissues with DAB staining.Immunofluorescence
Immunofluorescent staining of (A) HL-7702 and (B) SK-OV-3 cell line with antibody (green). DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.Enzyme-linked Immunoabsorbent Assay
ELISA analysis of ARF1 monoclonal antibody.Flow Cytometry
Flow cytometry analysis of HeLa cells using ARF1 mouse monoclonal antibody (green) and negative control (red). -
Gene Info — ARF1
Entrez GeneID
375Gene Name
ARF1
Gene Alias
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Gene Description
ADP-ribosylation factor 1
Omim ID
103180Gene Ontology
HyperlinkGene Summary
ADP-ribosylation factor 1 (ARF1) is a member of the human ARF gene family. The family members encode small guanine nucleotide-binding proteins that stimulate the ADP-ribosyltransferase activity of cholera toxin and play a role in vesicular trafficking as activators of phospholipase D. The gene products, including 6 ARF proteins and 11 ARF-like proteins, constitute a family of the RAS superfamily. The ARF proteins are categorized as class I (ARF1, ARF2 and ARF3), class II (ARF4 and ARF5) and class III (ARF6), and members of each class share a common gene organization. The ARF1 protein is localized to the Golgi apparatus and has a central role in intra-Golgi transport. Multiple alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq
Other Designations
OTTHUMP00000035715
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