YWHAB monoclonal antibody, clone 5B5G10
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More Files
- More Functions
Western Blot
Western Blot analysis of human YWHAB recombinant protein.
Western Blot
Western Blot analysis of (1) HeLa, (2) NIH/3T3, (3) C6, (4) A431, (5) K562, (6) PC-12, and (7) U937 cell lysate.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of (A) rectum cancer tissues and (B) endometrial cancer tissues with DAB staining.
Immunofluorescence
Immunofluorescent staining of (A) GC-7901 (B) HeLa and (C) HepG2 cell line with antibody (green). DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Enzyme-linked Immunoabsorbent Assay
ELISA analysis of YWHAB monoclonal antibody.
Flow Cytometry
Flow cytometric analysis of HeLa cells using YWHAB mouse monoclonal antibody (green) and negative control (red).
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Specification
Product Description
Mouse monoclonal antibody raised against recombinant human YWHAB.
Immunogen
Recombinant protein corresponding to amino acids 1-246 of of human YWHAB from E. coli.
Host
Mouse
Theoretical MW (kDa)
28
Reactivity
Human, Mouse, Rat
Form
Liquid
Isotype
IgG2b
Recommend Usage
ELISA (1:10000)
Flow Cytometry (1:200-400)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:200-1000)
Immunofluorescence (1:200-1000)
Western Blot (1:500-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.05% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western Blot analysis of human YWHAB recombinant protein.Western Blot
Western Blot analysis of (1) HeLa, (2) NIH/3T3, (3) C6, (4) A431, (5) K562, (6) PC-12, and (7) U937 cell lysate.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of (A) rectum cancer tissues and (B) endometrial cancer tissues with DAB staining.Immunofluorescence
Immunofluorescent staining of (A) GC-7901 (B) HeLa and (C) HepG2 cell line with antibody (green). DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.Enzyme-linked Immunoabsorbent Assay
ELISA analysis of YWHAB monoclonal antibody.Flow Cytometry
Flow cytometric analysis of HeLa cells using YWHAB mouse monoclonal antibody (green) and negative control (red). -
Gene Info — YWHAB
Entrez GeneID
7529Gene Name
YWHAB
Gene Alias
GW128, HS1, KCIP-1
Gene Description
tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, beta polypeptide
Omim ID
601289Gene Ontology
HyperlinkGene Summary
This gene encodes a protein belonging to the 14-3-3 family of proteins, members of which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals. The encoded protein has been shown to interact with RAF1 and CDC25 phosphatases, suggesting that it may play a role in linking mitogenic signaling and the cell cycle machinery. Two transcript variants, which encode the same protein, have been identified for this gene. [provided by RefSeq
Other Designations
14-3-3 protein beta/alpha|OTTHUMP00000031075|OTTHUMP00000031076|brain protein 14-3-3, beta isoform|protein 1054|protein kinase C inhibitor protein-1
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Interactome
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Pathway
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Disease
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