HDAC6 monoclonal antibody, clone 4G6F9
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Specification
Product Description
Mouse monoclonal antibody raised against recombinant human HDAC6.
Immunogen
Recombinant protein corresponding to amino acids 482-800 of of human HDAC6 from E. coli.
Host
Mouse
Theoretical MW (kDa)
131.4
Reactivity
Human
Form
Liquid
Isotype
IgG2b
Recommend Usage
ELISA (1:10000)
Flow Cytometry (1:200-400)
Western Blot (1:500-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.05% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western Blot analysis of human HDAC6 recombinant protein.Western Blot
Western Blot analysis of (1) HEK293 cells, (2) HDAC6-hIgGFc transfected HEK293 cell lysate.Enzyme-linked Immunoabsorbent Assay
ELISA analysis of HDAC6 monoclonal antibody.Flow Cytometry
Flow cytometric analysis of HeLa cells using HDAC6 mouse monoclonal antibody (green) and negative control (red). -
Gene Info — HDAC6
Entrez GeneID
10013Gene Name
HDAC6
Gene Alias
FLJ16239, HD6, JM21
Gene Description
histone deacetylase 6
Omim ID
300272Gene Ontology
HyperlinkGene Summary
Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene belongs to class II of the histone deacetylase/acuc/apha family. It contains an internal duplication of two catalytic domains which appear to function independently of each other. This protein possesses histone deacetylase activity and represses transcription. [provided by RefSeq
Other Designations
OTTHUMP00000032398
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Interactome
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Disease
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