RAB5A monoclonal antibody, clone 2E8B11
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More Files
- More Functions
Western Blot
Western Blot analysis of (1) K562, (2) HeLa, (3) Jurkat, and (4) HepG2 cell lysate.
Western Blot
Western Blot analysis of human RAB5A recombinant protein.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of ovarian cancer tissues with DAB staining.
Immunofluorescence
Immunofluorescent staining of HeLa cell line with antibody (green). DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Enzyme-linked Immunoabsorbent Assay
ELISA analysis of RAB5A monoclonal antibody.
Flow Cytometry
Flow cytometric analysis of HeLa cells using RAB5A mouse monoclonal antibody (green) and negative control (red).
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Specification
Product Description
Mouse monoclonal antibody raised against recombinant human RAB5A.
Immunogen
Recombinant protein corresponding to amino acids 1-215 of of human RAB5A from E. coli.
Host
Mouse
Theoretical MW (kDa)
23.7
Reactivity
Human
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Flow Cytometry (1:200-400)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:200-1000)
Immunofluorescence (1:200-1000)
Western Blot (1:500-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.05% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western Blot analysis of (1) K562, (2) HeLa, (3) Jurkat, and (4) HepG2 cell lysate.Western Blot
Western Blot analysis of human RAB5A recombinant protein.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of ovarian cancer tissues with DAB staining.Immunofluorescence
Immunofluorescent staining of HeLa cell line with antibody (green). DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.Enzyme-linked Immunoabsorbent Assay
ELISA analysis of RAB5A monoclonal antibody.Flow Cytometry
Flow cytometric analysis of HeLa cells using RAB5A mouse monoclonal antibody (green) and negative control (red). -
Gene Info — RAB5A
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Interactome
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Pathway
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Disease
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