MECP2 monoclonal antibody, clone 8H4A5B9
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More Files
- More Functions
Western Blot
Western Blot analysis of (1) A431, and (2) MCF-7 cell lysate.
Western Blot
Western Blot analysis of human MECP2 recombinant protein.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of (A) ovarian cancer tissues and (B) rectum cancer tissues with DAB staining.
Immunofluorescence
Immunofluorescent staining of HeLa cell line with antibody (green). DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Enzyme-linked Immunoabsorbent Assay
ELISA analysis of MECP2 monoclonal antibody.
Flow Cytometry
Flow cytometric analysis of HeLa cells using MECP2 mouse monoclonal antibody (green) and negative control (red).
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Specification
Product Description
Mouse monoclonal antibody raised against recombinant human MECP2.
Immunogen
Recombinant protein corresponding to amino acids 7-148 of of human MECP2 from E. coli.
Host
Mouse
Theoretical MW (kDa)
52.4
Reactivity
Human
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Flow Cytometry (1:200-400)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:200-1000)
Immunofluorescence (1:200-1000)
Western Blot (1:500-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.05% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western Blot analysis of (1) A431, and (2) MCF-7 cell lysate.Western Blot
Western Blot analysis of human MECP2 recombinant protein.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of (A) ovarian cancer tissues and (B) rectum cancer tissues with DAB staining.Immunofluorescence
Immunofluorescent staining of HeLa cell line with antibody (green). DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.Enzyme-linked Immunoabsorbent Assay
ELISA analysis of MECP2 monoclonal antibody.Flow Cytometry
Flow cytometric analysis of HeLa cells using MECP2 mouse monoclonal antibody (green) and negative control (red). -
Gene Info — MECP2
Entrez GeneID
4204Gene Name
MECP2
Gene Alias
AUTSX3, DKFZp686A24160, MRX16, MRX79, MRXS13, MRXSL, PPMX, RTS, RTT
Gene Description
methyl CpG binding protein 2 (Rett syndrome)
Gene Ontology
HyperlinkGene Summary
DNA methylation is the major modification of eukaryotic genomes and plays an essential role in mammalian development. Human proteins MECP2, MBD1, MBD2, MBD3, and MBD4 comprise a family of nuclear proteins related by the presence in each of a methyl-CpG binding domain (MBD). Each of these proteins, with the exception of MBD3, is capable of binding specifically to methylated DNA. MECP2, MBD1 and MBD2 can also repress transcription from methylated gene promoters. In contrast to other MBD family members, MECP2 is X-linked and subject to X inactivation. MECP2 is dispensible in stem cells, but is essential for embryonic development. MECP2 gene mutations are the cause of most cases of Rett syndrome, a progressive neurologic developmental disorder and one of the most common causes of mental retardation in females. [provided by RefSeq
Other Designations
OTTHUMP00000026021|methyl CpG binding protein 2
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Interactome
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Disease
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