DDX20 monoclonal antibody, clone 7B2B1
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More Files
- More Functions
Western Blot
Western Blot analysis of (1) HEK293 cells, (2) DDX20-hIgGFc transfected HEK293 cell lysate.
Western Blot
Western Blot analysis of human DDX20 recombinant protein.
Immunofluorescence
Immunofluorescent staining of HeLa cell line with antibody (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Enzyme-linked Immunoabsorbent Assay
ELISA analysis of DDX20 monoclonal antibody.
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Specification
Product Description
Mouse monoclonal antibody raised against recombinant human DDX20.
Immunogen
Recombinant protein corresponding to amino acids 725-824 of of human DDX20 from E. coli.
Host
Mouse
Theoretical MW (kDa)
92.2
Reactivity
Human
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Immunofluorescence (1:200-1000)
Western Blot (1:500-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.05% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western Blot analysis of (1) HEK293 cells, (2) DDX20-hIgGFc transfected HEK293 cell lysate.Western Blot
Western Blot analysis of human DDX20 recombinant protein.Immunofluorescence
Immunofluorescent staining of HeLa cell line with antibody (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.Enzyme-linked Immunoabsorbent Assay
ELISA analysis of DDX20 monoclonal antibody. -
Gene Info — DDX20
Entrez GeneID
11218Gene Name
DDX20
Gene Alias
DKFZp434H052, DP103, GEMIN3
Gene Description
DEAD (Asp-Glu-Ala-Asp) box polypeptide 20
Omim ID
606168Gene Ontology
HyperlinkGene Summary
DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein, which has an ATPase activity and is a component of the survival of motor neurons (SMN) complex. This protein interacts directly with SMN, the spinal muscular atrophy gene product, and may play a catalytic role in the function of the SMN complex on RNPs. [provided by RefSeq
Other Designations
DEAD-box protein DP103|DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 20, 103kD|OTTHUMP00000013738|SMN-interacting protein
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Interactome
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Disease
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