ATF4 monoclonal antibody, clone 2A6F12
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of Lane 1: K562 cell; Lane 2: A431 cell; Lane 3: HeLa cell; Lane 4: HEK293 cell; Lane 5: Ramos cell with ATF4 monoclonal antibody.
Western Blot (Transfected lysate)
Western Blot analysis of (1) HEK293 cells, (2) ATF4-hIgGFc transfected HEK293 cell lysate.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of paraffin-embedded breast cancer tissues with ATF4 monoclonal antibody.
Enzyme-linked Immunoabsorbent Assay
ELISA analysis of ATF4 monoclonal antibody, clone 2A6F12.
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Specification
Product Description
Mouse monoclonal antibody raised against recombinant human ATF4.
Immunogen
Recombinant protein corresponding to amino acid 212-351 of human ATF4 from E. coli.
Host
Mouse
Theoretical MW (kDa)
38.6kDa
Reactivity
Human
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Western Blot (1:500-1:2000)
Immunohistochemistry (1:200-1:1000)
Immunocytochemistry
Flow Cytometry
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.05% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of Lane 1: K562 cell; Lane 2: A431 cell; Lane 3: HeLa cell; Lane 4: HEK293 cell; Lane 5: Ramos cell with ATF4 monoclonal antibody.Western Blot (Transfected lysate)
Western Blot analysis of (1) HEK293 cells, (2) ATF4-hIgGFc transfected HEK293 cell lysate.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of paraffin-embedded breast cancer tissues with ATF4 monoclonal antibody.Enzyme-linked Immunoabsorbent Assay
ELISA analysis of ATF4 monoclonal antibody, clone 2A6F12. -
Gene Info — ATF4
Entrez GeneID
468Gene Name
ATF4
Gene Alias
CREB-2, CREB2, TAXREB67, TXREB
Gene Description
activating transcription factor 4 (tax-responsive enhancer element B67)
Omim ID
604064Gene Ontology
HyperlinkGene Summary
This gene encodes a transcription factor that was originally identified as a widely expressed mammalian DNA binding protein that could bind a tax-responsive enhancer element in the LTR of HTLV-1. The encoded protein was also isolated and characterized as the cAMP-response element binding protein 2 (CREB-2). The protein encoded by this gene belongs to a family of DNA-binding proteins that includes the AP-1 family of transcription factors, cAMP-response element binding proteins (CREBs) and CREB-like proteins. These transcription factors share a leucine zipper region that is involved in protein-protein interactions, located C-terminal to a stretch of basic amino acids that functions as a DNA binding domain. Two alternative transcripts encoding the same protein have been described. Two pseudogenes are located on the X chromsome at q28 in a region containing a large inverted duplication. [provided by RefSeq
Other Designations
activating transcription factor 4|cAMP response element-binding protein 2
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Interactome
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Pathway
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Disease
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