CDC37 monoclonal antibody, clone 6B3B7
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of Lane 1: K562 cell; Lane 2: LNcap cell; Lane 3: A431 cell; Lane 4: HEK293 cell; Lane 5: C2C12 cell with CDC37 monoclonal antibody.
Western Blot (Transfected lysate)
Western Blot analysis of (1) HEK293 cells, (2) CDC37-hIgGFc transfected HEK293 cell lysate.
Enzyme-linked Immunoabsorbent Assay
ELISA analysis of CDC37 monoclonal antibody, clone 6B3B7.
Flow Cytometry
Flow cytometric analysis of K562 cells with CDC37 monoclonal antibody (green) and negative control (red).
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Specification
Product Description
Mouse monoclonal antibody raised against recombinant human CDC37.
Immunogen
Recombinant protein corresponding to amino acid 241-378 of human CDC37 from E. coli.
Host
Mouse
Theoretical MW (kDa)
44.5kDa
Reactivity
Human, Mouse
Form
Liquid
Isotype
IgG2a
Recommend Usage
ELISA (1:10000)
Western Blot (1:500-1:2000)
Immunohistochemistry
Immunocytochemistry
Flow Cytometry (1:200-1:400)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.05% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of Lane 1: K562 cell; Lane 2: LNcap cell; Lane 3: A431 cell; Lane 4: HEK293 cell; Lane 5: C2C12 cell with CDC37 monoclonal antibody.Western Blot (Transfected lysate)
Western Blot analysis of (1) HEK293 cells, (2) CDC37-hIgGFc transfected HEK293 cell lysate.Enzyme-linked Immunoabsorbent Assay
ELISA analysis of CDC37 monoclonal antibody, clone 6B3B7.Flow Cytometry
Flow cytometric analysis of K562 cells with CDC37 monoclonal antibody (green) and negative control (red). -
Gene Info — CDC37
Entrez GeneID
11140Gene Name
CDC37
Gene Alias
P50CDC37
Gene Description
cell division cycle 37 homolog (S. cerevisiae)
Omim ID
605065Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is highly similar to Cdc 37, a cell division cycle control protein of Sacchromyces cerevisiae. This protein is a molecular chaperone with specific function in cell signal transduction. It has been shown to form complex with Hsp90 and a variety of protein kinases including CDK4, CDK6, SRC, RAF-1, MOK, as well as eIF2 alpha kinases. It is thought to play a critical role in directing Hsp90 to its target kinases. [provided by RefSeq
Other Designations
CDC37 (cell division cycle 37, S. cerevisiae, homolog)|CDC37 cell division cycle 37 homolog|Hsp90 co-chaperone Cdc37|cell division cycle 37 protein
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Interactome
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Disease
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