ESR1 monoclonal antibody, clone 6A6E8
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of Lane 1: MOLT4 cell; Lane 2: Raji cell; Lane 3: MCF-7 cell; Lane 4: T47D cell; Lane 5: SK-Br-3 cell; Lane 6: HeLa cell; Lane 7: C6 cell with ESR1 monoclonal antibody.
Western Blot (Transfected lysate)
Western Blot analysis of (1) HEK293 cells, (2) ESR1-hIgGFc transfected HEK293 cell lysate.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of paraffin-embedded breast cancer tissues with ESR1 monoclonal antibody.
Immunocytochemistry
Immunocytochemical staining of HeLa cells with ESR1 monoclonal antibody (green). DRAQ5 fluorescent DNA dye (blue). Actin filaments have been labeled with Alexa Fluor- 555 phalloidin (red).
Enzyme-linked Immunoabsorbent Assay
ELISA analysis of ESR1 monoclonal antibody, clone 6A6E8.
Flow Cytometry
Flow cytometric analysis of HeLa cells with ESR1 monoclonal antibody (green) and negative control (red).
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Specification
Product Description
Mouse monoclonal antibody raised against recombinant human ESR1.
Immunogen
Recombinant protein corresponding to amino acid 2-185 of human ESR1 from E. coli.
Host
Mouse
Theoretical MW (kDa)
66.2kDa
Reactivity
Human, Rat
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Western Blot (1:500-1:2000)
Immunohistochemistry (1:200-1:1000)
Immunocytochemistry (1:200-1:1000)
Flow Cytometry (1:200-1:400)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.05% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of Lane 1: MOLT4 cell; Lane 2: Raji cell; Lane 3: MCF-7 cell; Lane 4: T47D cell; Lane 5: SK-Br-3 cell; Lane 6: HeLa cell; Lane 7: C6 cell with ESR1 monoclonal antibody.Western Blot (Transfected lysate)
Western Blot analysis of (1) HEK293 cells, (2) ESR1-hIgGFc transfected HEK293 cell lysate.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of paraffin-embedded breast cancer tissues with ESR1 monoclonal antibody.Immunocytochemistry
Immunocytochemical staining of HeLa cells with ESR1 monoclonal antibody (green). DRAQ5 fluorescent DNA dye (blue). Actin filaments have been labeled with Alexa Fluor- 555 phalloidin (red).Enzyme-linked Immunoabsorbent Assay
ELISA analysis of ESR1 monoclonal antibody, clone 6A6E8.Flow Cytometry
Flow cytometric analysis of HeLa cells with ESR1 monoclonal antibody (green) and negative control (red). -
Gene Info — ESR1
Entrez GeneID
2099Gene Name
ESR1
Gene Alias
DKFZp686N23123, ER, ESR, ESRA, Era, NR3A1
Gene Description
estrogen receptor 1
Gene Ontology
HyperlinkGene Summary
This gene encodes an estrogen receptor, a ligand-activated transcription factor composed of several domains important for hormone binding, DNA binding, and activation of transcription. The protein localizes to the nucleus where it may form a homodimer or a heterodimer with estrogen receptor 2. Estrogen and its receptors are essential for sexual development and reproductive function, but also play a role in other tissues such as bone. Estrogen receptors are also involved in pathological processes including breast cancer, endometrial cancer, and osteoporosis. Alternative splicing results in several transcript variants, which differ in their 5' UTRs and use different promoters. [provided by RefSeq
Other Designations
OTTHUMP00000017718|OTTHUMP00000017719|estrogen receptor alpha
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Interactome
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Disease
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