CCND1 monoclonal antibody, clone 3B6G3
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Specification
Product Description
Mouse monoclonal antibody raised against recombinant human CCND1.
Immunogen
Recombinant protein corresponding to amino acid 167-295 of human CCND1 from E. coli.
Host
Mouse
Theoretical MW (kDa)
33.7kDa
Reactivity
Human
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Western Blot (1:500-1:2000)
Immunohistochemistry (1:200-1:1000)
Immunocytochemistry (1:100-1:500)
Flow Cytometry (1:200-1:400)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.05% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western Blot analysis of (1) HEK293 cells, (2) CCND1-hIgGFc transfected HEK293 cell lysate.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of paraffin-embedded bladder cancer tissues with CCND1 monoclonal antibody.Immunocytochemistry
Immunocytochemical staining of HeLa cells with CCND1 monoclonal antibody (green). DRAQ5 fluorescent DNA dye (blue). Actin filaments have been labeled with Alexa Fluor- 555 phalloidin (red).Enzyme-linked Immunoabsorbent Assay
ELISA analysis of CCND1 monoclonal antibody, clone 3B6G3.Flow Cytometry
Flow cytometric analysis of MCF-7 cells with CCND1 monoclonal antibody (green) and negative control (red). -
Gene Info — CCND1
Entrez GeneID
595Gene Name
CCND1
Gene Alias
BCL1, D11S287E, PRAD1, U21B31
Gene Description
cyclin D1
Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance throughout the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activity is required for cell cycle G1/S transition. This protein has been shown to interact with tumor suppressor protein Rb and the expression of this gene is regulated positively by Rb. Mutations, amplification and overexpression of this gene, which alters cell cycle progression, are observed frequently in a variety of tumors and may contribute to tumorigenesis. [provided by RefSeq
Other Designations
B-cell CLL/lymphoma 1|G1/S-specific cyclin D1
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Interactome
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Pathway
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Disease
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