CPV-VP2 monoclonal antibody, clone 2G10A3
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Specification
Product Description
Mouse monoclonal antibody raised against recombinant Canine Parvovirus CPV-VP2.
Immunogen
Recombinant protein corresponding to amino acid 1-128 of Canine Parvovirus CPV-VP2 from E. coli.
Host
Mouse
Theoretical MW (kDa)
64.6kDa
Reactivity
Human
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Western Blot (1:500-1:2000)
Immunohistochemistry (1:200-1:1000)
Immunocytochemistry (1:200-1:1000)
Flow Cytometry (1:200-1:400)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.05% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of Lane 1: SW480 cell; Lane 2: A431 cell; Lane 3: K562 cell; Lane 4: BCBL-1 cell with CPV-VP2 monoclonal antibody.Western Blot (Transfected lysate)
Western Blot analysis of (1) HEK293 cells, (2) CPV-VP2-hIgGFc transfected HEK293 cell lysate.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of paraffin-embedded cervical cancer tissues with CPV-VP2 monoclonal antibody.Immunocytochemistry
Immunocytochemical staining of HeLa cells with CPV-VP2 monoclonal antibody (green). DRAQ5 fluorescent DNA dye (blue). Actin filaments have been labeled with Alexa Fluor- 555 phalloidin (red).Enzyme-linked Immunoabsorbent Assay
ELISA analysis of CPV-VP2 monoclonal antibody, clone 2G10A3.Flow Cytometry
Flow cytometric analysis of HeLa cells with CPV-VP2 monoclonal antibody (green) and negative control (red).
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