IDH1 monoclonal antibody, clone 4A4A8
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of Lane 1: HepG2 cell; Lane 2: NIH/3T3 cell; Lane 3: C2C12 cell; Lane 4: COS-7 cell; Lane 5: SW480 cell with IDH1 monoclonal antibody.
Western Blot (Transfected lysate)
Western Blot analysis of (1) HEK293 cells, (2) IDH1-hIgGFc transfected HEK293 cell lysate.
Enzyme-linked Immunoabsorbent Assay
ELISA analysis of IDH1 monoclonal antibody, clone 4A4A8.
Flow Cytometry
Flow cytometric analysis of HeLa cells with IDH1 monoclonal antibody (green) and negative control (red).
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Specification
Product Description
Mouse monoclonal antibody raised against recombinant human IDH1.
Immunogen
Recombinant protein corresponding to amino acid 156-298 of human IDH1 from E. coli.
Host
Mouse
Theoretical MW (kDa)
46.7kDa
Reactivity
Human, Monkey, Mouse
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Western Blot (1:500-1:2000)
Immunohistochemistry
Immunocytochemistry
Flow Cytometry (1:200-1:400)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.05% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of Lane 1: HepG2 cell; Lane 2: NIH/3T3 cell; Lane 3: C2C12 cell; Lane 4: COS-7 cell; Lane 5: SW480 cell with IDH1 monoclonal antibody.Western Blot (Transfected lysate)
Western Blot analysis of (1) HEK293 cells, (2) IDH1-hIgGFc transfected HEK293 cell lysate.Enzyme-linked Immunoabsorbent Assay
ELISA analysis of IDH1 monoclonal antibody, clone 4A4A8.Flow Cytometry
Flow cytometric analysis of HeLa cells with IDH1 monoclonal antibody (green) and negative control (red). -
Gene Info — IDH1
Entrez GeneID
3417Gene Name
IDH1
Gene Alias
IDCD, IDH, IDP, IDPC, PICD
Gene Description
isocitrate dehydrogenase 1 (NADP+), soluble
Omim ID
147700Gene Ontology
HyperlinkGene Summary
Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. Each NADP(+)-dependent isozyme is a homodimer. The protein encoded by this gene is the NADP(+)-dependent isocitrate dehydrogenase found in the cytoplasm and peroxisomes. It contains the PTS-1 peroxisomal targeting signal sequence. The presence of this enzyme in peroxisomes suggests roles in the regeneration of NADPH for intraperoxisomal reductions, such as the conversion of 2, 4-dienoyl-CoAs to 3-enoyl-CoAs, as well as in peroxisomal reactions that consume 2-oxoglutarate, namely the alpha-hydroxylation of phytanic acid. The cytoplasmic enzyme serves a significant role in cytoplasmic NADPH production. [provided by RefSeq
Other Designations
NADP+-specific ICDH|NADP-dependent isocitrate dehydrogenase, cytosolic|NADP-dependent isocitrate dehydrogenase, peroxisomal|oxalosuccinate decarboxylase
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Interactome
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Pathway
- Biosynthesis of alkaloids derived from histidine and purine
- Biosynthesis of alkaloids derived from ornithine
- Biosynthesis of alkaloids derived from shikimate pathway
- Biosynthesis of alkaloids derived from terpenoid and polyketide
- Biosynthesis of phenylpropanoids
- Biosynthesis of plant hormones
- Biosynthesis of terpenoids and steroids
- Citrate cycle (TCA cycle)
- Glutathione metabolism
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Disease
- Adenoma
- Astrocytoma
- Blast Crisis
- Brain Neoplasms
- Carcinoma
- Chronic Disease
- Cleft Lip
- Cleft Palate
- Disease Progression
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