SOD2 monoclonal antibody, clone 8H3D2
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More Files
- More Functions
Western Blot (Transfected lysate)
Western Blot analysis of (1) HEK293 cells, (2) SOD2-hIgGFc transfected HEK293 cell lysate.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of paraffin-embedded ovarian cancer tissues with SOD2 monoclonal antibody.
Immunocytochemistry
Immunocytochemical staining of HeLa cells with SOD2 monoclonal antibody (green). DRAQ5 fluorescent DNA dye (blue). Actin filaments have been labeled with Alexa Fluor- 555 phalloidin (red).
Enzyme-linked Immunoabsorbent Assay
ELISA analysis of SOD2 monoclonal antibody, clone 8H3D2.
Flow Cytometry
Flow cytometric analysis of HeLa cells with SOD2 monoclonal antibody (green) and negative control (red).
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Specification
Product Description
Mouse monoclonal antibody raised against recombinant human SOD2.
Immunogen
Recombinant protein corresponding to amino acid 1-222 of human SOD2 from E. coli.
Host
Mouse
Theoretical MW (kDa)
24.7kDa
Reactivity
Human
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Western Blot (1:500-1:2000)
Immunohistochemistry (1:200-1:1000)
Immunocytochemistry (1:50-1:250)
Flow Cytometry (1:200-1:400)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.05% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western Blot analysis of (1) HEK293 cells, (2) SOD2-hIgGFc transfected HEK293 cell lysate.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of paraffin-embedded ovarian cancer tissues with SOD2 monoclonal antibody.Immunocytochemistry
Immunocytochemical staining of HeLa cells with SOD2 monoclonal antibody (green). DRAQ5 fluorescent DNA dye (blue). Actin filaments have been labeled with Alexa Fluor- 555 phalloidin (red).Enzyme-linked Immunoabsorbent Assay
ELISA analysis of SOD2 monoclonal antibody, clone 8H3D2.Flow Cytometry
Flow cytometric analysis of HeLa cells with SOD2 monoclonal antibody (green) and negative control (red). -
Gene Info — SOD2
Entrez GeneID
6648Gene Name
SOD2
Gene Alias
IPO-B, MNSOD, Mn-SOD
Gene Description
superoxide dismutase 2, mitochondrial
Omim ID
147460Gene Ontology
HyperlinkGene Summary
This gene is a member of the iron/manganese superoxide dismutase family. It encodes a mitochondrial protein that forms a homotetramer and binds one manganese ion per subunit. This protein binds to the superoxide byproducts of oxidative phosphorylation and converts them to hydrogen peroxide and diatomic oxygen. Mutations in this gene have been associated with idiopathic cardiomyopathy (IDC), premature aging, sporadic motor neuron disease, and cancer. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq
Other Designations
Mn superoxide dismutase|OTTHUMP00000017530|OTTHUMP00000017531|indophenoloxidase B|manganese superoxide dismutase|manganese-containing superoxide dismutase|mangano-superoxide dismutase
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Interactome
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Disease
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