BNIP3 monoclonal antibody, clone 4E11F4
* The price is valid only in USA. Please select country.
-
More Files
- More Functions
Western Blot (Transfected lysate)
Western Blot analysis of (1) HEK293 cells, (2) BNIP3-hIgGFc transfected HEK293 cell lysate.
Immunocytochemistry
Immunocytochemical staining of HeLa cells with BNIP3 monoclonal antibody (green). DRAQ5 fluorescent DNA dye (blue). Actin filaments have been labeled with Alexa Fluor- 555 phalloidin (red).
Enzyme-linked Immunoabsorbent Assay
ELISA analysis of BNIP3 monoclonal antibody, clone 4E11F4.
Flow Cytometry
Flow cytometric analysis of HeLa cells with BNIP3 monoclonal antibody (green) and negative control (red).
-
Specification
Product Description
Mouse monoclonal antibody raised against recombinant human BNIP3.
Immunogen
Recombinant protein corresponding to amino acid 50-155 of human BNIP3 from E. coli.
Host
Mouse
Theoretical MW (kDa)
21.5kDa
Reactivity
Human
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Western Blot (1:500-1:2000)
Immunohistochemistry
Immunocytochemistry (1:200-1:1000)
Flow Cytometry (1:200-1:400)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.05% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
Applications
Western Blot (Transfected lysate)
Western Blot analysis of (1) HEK293 cells, (2) BNIP3-hIgGFc transfected HEK293 cell lysate.Immunocytochemistry
Immunocytochemical staining of HeLa cells with BNIP3 monoclonal antibody (green). DRAQ5 fluorescent DNA dye (blue). Actin filaments have been labeled with Alexa Fluor- 555 phalloidin (red).Enzyme-linked Immunoabsorbent Assay
ELISA analysis of BNIP3 monoclonal antibody, clone 4E11F4.Flow Cytometry
Flow cytometric analysis of HeLa cells with BNIP3 monoclonal antibody (green) and negative control (red). -
Gene Info — BNIP3
Entrez GeneID
664Gene Name
BNIP3
Gene Alias
NIP3
Gene Description
BCL2/adenovirus E1B 19kDa interacting protein 3
Omim ID
603293Gene Ontology
HyperlinkGene Summary
This gene is a member of the BCL2/adenovirus E1B 19 kd-interacting protein (BNIP) family. It interacts with the E1B 19 kDa protein which is responsible for the protection of virally-induced cell death, as well as E1B 19 kDa-like sequences of BCL2, also an apoptotic protector. This gene contains a BH3 domain and a transmembrane domain, which have been associated with pro-apoptotic function. The dimeric mitochondrial protein encoded by this gene is known to induce apoptosis, even in the presence of BCL2. [provided by RefSeq
Other Designations
BCL2/adenovirus E1B 19kD-interacting protein 3|OTTHUMP00000020752
-
Interactome
- +1-909-264-1399
+1-909-992-0619
Toll Free : +1-877-853-6098 - +1-909-992-3401
- sales@abnova.com