DDX58 monoclonal antibody, clone 4G1B6
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Specification
Product Description
Mouse monoclonal antibody raised against recombinant human DDX58.
Immunogen
Recombinant protein corresponding to amino acid 789-925 of human DDX58 from E. coli.
Host
Mouse
Theoretical MW (kDa)
106.6kDa
Reactivity
Human
Form
Liquid
Isotype
IgM
Recommend Usage
ELISA (1:10000)
Western Blot (1:500-1:2000)
Immunohistochemistry
Immunocytochemistry (1:200-1:1000)
Flow Cytometry (1:200-1:400)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.05% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western Blot analysis of (1) HEK293 cells, (2) DDX58-hIgGFc transfected HEK293 cell lysate.Immunocytochemistry
Immunocytochemical staining of HeLa cells with DDX58 monoclonal antibody (green). DRAQ5 fluorescent DNA dye (blue). Actin filaments have been labeled with Alexa Fluor- 555 phalloidin (red).Enzyme-linked Immunoabsorbent Assay
ELISA analysis of DDX58 monoclonal antibody, clone 4G1B6.Flow Cytometry
Flow cytometric analysis of HeLa cells with DDX58 monoclonal antibody (green) and negative control (red). -
Gene Info — DDX58
Entrez GeneID
23586Gene Name
DDX58
Gene Alias
DKFZp434J1111, DKFZp686N19181, FLJ13599, RIG-I
Gene Description
DEAD (Asp-Glu-Ala-Asp) box polypeptide 58
Omim ID
609631Gene Ontology
HyperlinkGene Summary
DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases which are implicated in a number of cellular processes involving RNA binding and alteration of RNA secondary structure. This gene encodes a protein containing RNA helicase-DEAD box protein motifs and a caspase recruitment domain (CARD). It is involved in viral double-stranded (ds) RNA recognition and the regulation of immune response. [provided by RefSeq
Other Designations
DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide|DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide RIG-I|OTTHUMP00000021185|OTTHUMP00000045225|RNA helicase RIG-I|retinoic acid-inducible gene I
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Interactome
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Disease
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