DDX1 monoclonal antibody, clone 3E5B2
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of Lane 1: HeLa cell; Lane 2: MCF-7 cell; Lane 3: A431 cell; Lane 4: PC-3 cell; Lane 5: Jurkat cell with DDX1 monoclonal antibody.
Western Blot (Transfected lysate)
Western Blot analysis of (1) HEK293 cells, (2) DDX1-hIgGFc transfected HEK293 cell lysate.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
mmunohistochemical staining of paraffin-embedded cervical cancer tissues with DDX1 monoclonal antibody.
Immunocytochemistry
Immunocytochemical staining of HeLa cells with DDX1 monoclonal antibody (green). DRAQ5 fluorescent DNA dye (blue). Actin filaments have been labeled with Alexa Fluor- 555 phalloidin (red).
Enzyme-linked Immunoabsorbent Assay
ELISA analysis of DDX1 monoclonal antibody, clone 3E5B2.
Flow Cytometry
Flow cytometric analysis of HeLa cells with DDX1 monoclonal antibody (green) and negative control (red).
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Specification
Product Description
Mouse monoclonal antibody raised against recombinant human DDX1.
Immunogen
Recombinant protein corresponding to amino acid 642-740 of human DDX1 from E. coli.
Host
Mouse
Theoretical MW (kDa)
82.4kDa
Reactivity
Human
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Western Blot (1:500-1:2000)
Immunohistochemistry (1:200-1:1000)
Immunocytochemistry (1:200-1:1000)
Flow Cytometry (1:200-1:400)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.05% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of Lane 1: HeLa cell; Lane 2: MCF-7 cell; Lane 3: A431 cell; Lane 4: PC-3 cell; Lane 5: Jurkat cell with DDX1 monoclonal antibody.Western Blot (Transfected lysate)
Western Blot analysis of (1) HEK293 cells, (2) DDX1-hIgGFc transfected HEK293 cell lysate.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
mmunohistochemical staining of paraffin-embedded cervical cancer tissues with DDX1 monoclonal antibody.Immunocytochemistry
Immunocytochemical staining of HeLa cells with DDX1 monoclonal antibody (green). DRAQ5 fluorescent DNA dye (blue). Actin filaments have been labeled with Alexa Fluor- 555 phalloidin (red).Enzyme-linked Immunoabsorbent Assay
ELISA analysis of DDX1 monoclonal antibody, clone 3E5B2.Flow Cytometry
Flow cytometric analysis of HeLa cells with DDX1 monoclonal antibody (green) and negative control (red). -
Gene Info — DDX1
Entrez GeneID
1653Gene Name
DDX1
Gene Alias
DBP-RB, UKVH5d
Gene Description
DEAD (Asp-Glu-Ala-Asp) box polypeptide 1
Omim ID
601257Gene Ontology
HyperlinkGene Summary
DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein of unknown function. It shows high transcription levels in 2 retinoblastoma cell lines and in tissues of neuroectodermal origin. [provided by RefSeq
Other Designations
DEAD box polypeptide 1|DEAD box-1|DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 1|OTTHUMP00000115711
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Interactome
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Disease
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