PSMC3 monoclonal antibody, clone 1G10C9
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of Lane 1: MCF-7 cell; Lane 2: PC-3 cell; Lane 3: T47D cell; Lane 4: SW620 cell; Lane 5: COS-7 cell; Lane 6: C6 cell; Lane 7: HeLa cell; Lane 8: A431 cell with PSMC3 monoclonal antibody.
Western Blot (Transfected lysate)
Western Blot analysis of (1) HEK293 cells, (2) PSMC3-hIgGFc transfected HEK293 cell lysate.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of paraffin-embedded breast cancer tissues with PSMC3 monoclonal antibody.
Immunocytochemistry
Immunocytochemical staining of MCF-7 cells with PSMC3 monoclonal antibody (green). DRAQ5 fluorescent DNA dye (blue). Actin filaments have been labeled with Alexa Fluor- 555 phalloidin (red).
Enzyme-linked Immunoabsorbent Assay
ELISA analysis of PSMC3 monoclonal antibody, clone 1G10C9.
Flow Cytometry
Flow cytometric analysis of HeLa cells with PSMC3 monoclonal antibody (green) and negative control (red).
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Specification
Product Description
Mouse monoclonal antibody raised against recombinant human PSMC3.
Immunogen
Recombinant protein corresponding to amino acid 53-152 of human PSMC3 from E. coli.
Host
Mouse
Theoretical MW (kDa)
49kDa
Reactivity
Human, Monkey, Rat
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Western Blot (1:500-1:2000)
Immunohistochemistry (1:200-1:1000)
Immunocytochemistry (1:200-1:1000)
Flow Cytometry (1:200-1:400)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.05% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of Lane 1: MCF-7 cell; Lane 2: PC-3 cell; Lane 3: T47D cell; Lane 4: SW620 cell; Lane 5: COS-7 cell; Lane 6: C6 cell; Lane 7: HeLa cell; Lane 8: A431 cell with PSMC3 monoclonal antibody.Western Blot (Transfected lysate)
Western Blot analysis of (1) HEK293 cells, (2) PSMC3-hIgGFc transfected HEK293 cell lysate.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of paraffin-embedded breast cancer tissues with PSMC3 monoclonal antibody.Immunocytochemistry
Immunocytochemical staining of MCF-7 cells with PSMC3 monoclonal antibody (green). DRAQ5 fluorescent DNA dye (blue). Actin filaments have been labeled with Alexa Fluor- 555 phalloidin (red).Enzyme-linked Immunoabsorbent Assay
ELISA analysis of PSMC3 monoclonal antibody, clone 1G10C9.Flow Cytometry
Flow cytometric analysis of HeLa cells with PSMC3 monoclonal antibody (green) and negative control (red). -
Gene Info — PSMC3
Entrez GeneID
5702Gene Name
PSMC3
Gene Alias
MGC8487, TBP1
Gene Description
proteasome (prosome, macropain) 26S subunit, ATPase, 3
Omim ID
186852Gene Ontology
HyperlinkGene Summary
The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes one of the ATPase subunits, a member of the triple-A family of ATPases that have chaperone-like activity. This subunit may compete with PSMC2 for binding to the HIV tat protein to regulate the interaction between the viral protein and the transcription complex. A pseudogene has been identified on chromosome 9. [provided by RefSeq
Other Designations
Tat-binding protein 1|human immunodeficiency virus tat transactivator binding protein-1|proteasome 26S ATPase subunit 3|proteasome subunit P50
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Interactome
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Pathway
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