PGRMC1 monoclonal antibody, clone 7G11G8
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Specification
Product Description
Mouse monoclonal antibody raised against recombinant human PGRMC1.
Immunogen
Recombinant protein corresponding to amino acid 1-195 of human PGRMC1 from E. coli.
Host
Mouse
Theoretical MW (kDa)
21.7kDa
Reactivity
Human
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Western Blot (1:500-1:2000)
Immunohistochemistry (1:200-1:1000)
Immunocytochemistry (1:200-1:1000)
Flow Cytometry (1:200-1:400)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.05% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western Blot analysis of (1) HEK293 cells, (2) PGRMC1-hIgGFc transfected HEK293 cell lysate.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of paraffin-embedded cervical cancer tissues with PGRMC1 monoclonal antibody.Immunocytochemistry
Immunocytochemical staining of SK-OV-3 cells with PGRMC1 monoclonal antibody (green). DRAQ5 fluorescent DNA dye (blue). Actin filaments have been labeled with Alexa Fluor- 555 phalloidin (red).Enzyme-linked Immunoabsorbent Assay
ELISA analysis of PGRMC1 monoclonal antibody, clone 7G11G8.Flow Cytometry
Flow cytometric analysis of A549 cells with PGRMC1 monoclonal antibody (green) and negative control (red). -
Gene Info — PGRMC1
Entrez GeneID
10857Gene Name
PGRMC1
Gene Alias
HPR6.6, MPR
Gene Description
progesterone receptor membrane component 1
Omim ID
300435Gene Ontology
HyperlinkGene Summary
Progesterone binding protein is a putative steroid membrane receptor. The protein is expressed predominantly in the liver and kidney. [provided by RefSeq
Other Designations
OTTHUMP00000023897|progesterone binding protein
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