PYCARD monoclonal antibody, clone 1C3D7
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More Files
- More Functions
Western Blot (Transfected lysate)
Western Blot analysis of (1) HEK293 cells, (2) PYCARD-hIgGFc transfected HEK293 cell lysate.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of paraffin-embedded ovarian cancer tissues with PYCARD monoclonal antibody.
Immunocytochemistry
Immunocytochemical staining of GC-7901 cells with PYCARD monoclonal antibody (green). DRAQ5 fluorescent DNA dye (blue). Actin filaments have been labeled with Alexa Fluor- 555 phalloidin (red).
Enzyme-linked Immunoabsorbent Assay
ELISA analysis of PYCARD monoclonal antibody, clone 1C3D7.
Flow Cytometry
Flow cytometric analysis of HEK293 cells with PYCARD monoclonal antibody (green) and negative control (red).
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Specification
Product Description
Mouse monoclonal antibody raised against recombinant human PYCARD.
Immunogen
Recombinant protein corresponding to amino acid 1-120 of human PYCARD from E. coli.
Host
Mouse
Theoretical MW (kDa)
21.6kDa
Reactivity
Human
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Western Blot (1:500-1:2000)
Immunohistochemistry (1:200-1:1000)
Immunocytochemistry (1:200-1:1000)
Flow Cytometry (1:200-1:400)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.05% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western Blot analysis of (1) HEK293 cells, (2) PYCARD-hIgGFc transfected HEK293 cell lysate.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of paraffin-embedded ovarian cancer tissues with PYCARD monoclonal antibody.Immunocytochemistry
Immunocytochemical staining of GC-7901 cells with PYCARD monoclonal antibody (green). DRAQ5 fluorescent DNA dye (blue). Actin filaments have been labeled with Alexa Fluor- 555 phalloidin (red).Enzyme-linked Immunoabsorbent Assay
ELISA analysis of PYCARD monoclonal antibody, clone 1C3D7.Flow Cytometry
Flow cytometric analysis of HEK293 cells with PYCARD monoclonal antibody (green) and negative control (red). -
Gene Info — PYCARD
Entrez GeneID
29108Gene Name
PYCARD
Gene Alias
ASC, CARD5, MGC10332, TMS, TMS-1, TMS1
Gene Description
PYD and CARD domain containing
Omim ID
606838Gene Ontology
HyperlinkGene Summary
This gene encodes an adaptor protein that is composed of two protein-protein interaction domains: a N-terminal PYRIN-PAAD-DAPIN domain (PYD) and a C-terminal caspase-recruitment domain (CARD). The PYD and CARD domains are members of the six-helix bundle death domain-fold superfamily that mediates assembly of large signaling complexes in the inflammatory and apoptotic signaling pathways via the activation of caspase. In normal cells, this protein is localized to the cytoplasm; however, in cells undergoing apoptosis, it forms ball-like aggregates near the nuclear periphery. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
Other Designations
apoptosis-associated speck-like protein containing a CARD|caspase recruitment domain protein 5|target of methylation-induced silencing-1
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Interactome
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Disease
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