UCP3 monoclonal antibody, clone 6B8C6
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More Files
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Specification
Product Description
Mouse monoclonal antibody raised against recombinant human UCP3.
Immunogen
Recombinant protein corresponding to amino acid 1-113 and 217-312 of human UCP3 from E. coli.
Host
Mouse
Theoretical MW (kDa)
34.2kDa
Reactivity
Human
Form
Liquid
Isotype
IgG2a
Recommend Usage
ELISA (1:10000)
Western Blot (1:500-1:2000)
Immunohistochemistry
Immunocytochemistry (1:200-1:1000)
Flow Cytometry
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.05% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western Blot analysis of (1) HEK293 cells, (2) UCP3-hIgGFc transfected HEK293 cell lysate.Immunocytochemistry
Immunocytochemical staining of HL-7702 cells with UCP3 monoclonal antibody (green). DRAQ5 fluorescent DNA dye (blue). Actin filaments have been labeled with Alexa Fluor- 555 phalloidin (red).Enzyme-linked Immunoabsorbent Assay
ELISA analysis of UCP3 monoclonal antibody, clone 6B8C6. -
Gene Info — UCP3
Entrez GeneID
7352Gene Name
UCP3
Gene Alias
SLC25A9
Gene Description
uncoupling protein 3 (mitochondrial, proton carrier)
Gene Ontology
HyperlinkGene Summary
Mitochondrial uncoupling proteins (UCP) are members of the larger family of mitochondrial anion carrier proteins (MACP). UCPs separate oxidative phosphorylation from ATP synthesis with energy dissipated as heat, also referred to as the mitochondrial proton leak. UCPs facilitate the transfer of anions from the inner to the outer mitochondrial membrane and the return transfer of protons from the outer to the inner mitochondrial membrane. They also reduce the mitochondrial membrane potential in mammalian cells. The different UCPs have tissue-specific expression; this gene is primarily expressed in skeletal muscle. This gene's protein product is postulated to protect mitochondria against lipid-induced oxidative stress. Expression levels of this gene increase when fatty acid supplies to mitochondria exceed their oxidation capacity and the protein enables the export of fatty acids from mitochondria. UCPs contain the three solcar protein domains typically found in MACPs. Two splice variants have been found for this gene
Other Designations
Uncoupling protein-3|uncoupling protein 3
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Interactome
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Disease
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