RAN monoclonal antibody, clone 8D1A6
* The price is valid only in USA. Please select country.
-
More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of Lane 1: HeLa cell; Lane 2: NIH/3T3 cell; Lane 3: A431 cell; Lane 4: C6 cell; Lane 5: Jurkat cell; Lane 6: HeLa cell; Lane 7: COS-7 cell; Lane 8: Jurkat cell with RAN monoclonal antibody.
Western Blot (Transfected lysate)
Western Blot analysis of (1) HEK293 cells, (2) RAN-hIgGFc transfected HEK293 cell lysate.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of paraffin-embedded cervical cancer tissues with RAN monoclonal antibody.
Immunocytochemistry
Immunocytochemical staining of GC-7901 cells with RAN monoclonal antibody (green). DRAQ5 fluorescent DNA dye (blue). Actin filaments have been labeled with Alexa Fluor- 555 phalloidin (red).
Enzyme-linked Immunoabsorbent Assay
ELISA analysis of RAN monoclonal antibody, clone 8D1A6.
Flow Cytometry
Flow cytometric analysis of HeLa cells with RAN monoclonal antibody (green) and negative control (red).
-
Specification
Product Description
Mouse monoclonal antibody raised against recombinant human RAN.
Immunogen
Recombinant protein corresponding to amino acid 1-216 of human RAN from E. coli.
Host
Mouse
Theoretical MW (kDa)
24.4kDa
Reactivity
Human, Monkey, Mouse, Rat
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Western Blot (1:500-1:2000)
Immunohistochemistry (1:200-1:1000)
Immunocytochemistry (1:200-1:1000)
Flow Cytometry (1:200-1:400)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.05% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
Applications
Western Blot (Cell lysate)
Western blot analysis of Lane 1: HeLa cell; Lane 2: NIH/3T3 cell; Lane 3: A431 cell; Lane 4: C6 cell; Lane 5: Jurkat cell; Lane 6: HeLa cell; Lane 7: COS-7 cell; Lane 8: Jurkat cell with RAN monoclonal antibody.Western Blot (Transfected lysate)
Western Blot analysis of (1) HEK293 cells, (2) RAN-hIgGFc transfected HEK293 cell lysate.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of paraffin-embedded cervical cancer tissues with RAN monoclonal antibody.Immunocytochemistry
Immunocytochemical staining of GC-7901 cells with RAN monoclonal antibody (green). DRAQ5 fluorescent DNA dye (blue). Actin filaments have been labeled with Alexa Fluor- 555 phalloidin (red).Enzyme-linked Immunoabsorbent Assay
ELISA analysis of RAN monoclonal antibody, clone 8D1A6.Flow Cytometry
Flow cytometric analysis of HeLa cells with RAN monoclonal antibody (green) and negative control (red). -
Gene Info — RAN
Entrez GeneID
5901Gene Name
RAN
Gene Alias
ARA24, Gsp1, TC4
Gene Description
RAN, member RAS oncogene family
Omim ID
601179Gene Ontology
HyperlinkGene Summary
RAN (ras-related nuclear protein) is a small GTP binding protein belonging to the RAS superfamily that is essential for the translocation of RNA and proteins through the nuclear pore complex. The RAN protein is also involved in control of DNA synthesis and cell cycle progression. Nuclear localization of RAN requires the presence of regulator of chromosome condensation 1 (RCC1). Mutations in RAN disrupt DNA synthesis. Because of its many functions, it is likely that RAN interacts with several other proteins. RAN regulates formation and organization of the microtubule network independently of its role in the nucleus-cytosol exchange of macromolecules. RAN could be a key signaling molecule regulating microtubule polymerization during mitosis. RCC1 generates a high local concentration of RAN-GTP around chromatin which, in turn, induces the local nucleation of microtubules. RAN is an androgen receptor (AR) coactivator that binds differentially with different lengths of polyglutamine within the androgen receptor. Polyglutamine repeat expansion in the AR is linked to Kennedy's disease (X-linked spinal and bulbar muscular atrophy). RAN coactivation of the AR diminishes with polyglutamine expansion within the AR, and this weak coactivation may lead to partial androgen insensitivity during the development of Kennedy's disease. [provided by RefSeq
Other Designations
OK/SW-cl.81|RanGTPase|guanosine triphosphatase Ran|member RAS oncogene family|ras-related nuclear protein
-
Interactome
-
Disease
- +1-909-264-1399
+1-909-992-0619
Toll Free : +1-877-853-6098 - +1-909-992-3401
- sales@abnova.com