DDX5 monoclonal antibody, clone 4F1C12
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More Files
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Specification
Product Description
Mouse monoclonal antibody raised against recombinant human DDX5.
Immunogen
Recombinant protein corresponding to amino acid 475-614 of human DDX5 from E. coli.
Host
Mouse
Theoretical MW (kDa)
69.1kDa
Reactivity
Human, Monkey, Mouse
Form
Liquid
Isotype
IgG2a
Recommend Usage
ELISA (1:10000)
Western Blot (1:500-1:2000)
Immunohistochemistry (1:200-1:1000)
Immunocytochemistry
Flow Cytometry (1:200-1:400)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.05% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of Lane 1: HT-29 cell; Lane 2: HeLa cell; NIH/3T3 cell; Lane 3: COS-7 cell; Lane 4: SW620 cell; Lane 5: Jurkat cell; Lane 6: A431 cell; Lane 7: MCF-7 cell with DDX5 monoclonal antibody.Western Blot (Transfected lysate)
Western Blot analysis of (1) HEK293 cells, (2) DDX5-hIgGFc transfected HEK293 cell lysate.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of paraffin-embedded bladder cancer tissues with DDX5 monoclonal antibody.Enzyme-linked Immunoabsorbent Assay
ELISA analysis of DDX5 monoclonal antibody, clone 4F1C12.Flow Cytometry
Flow cytometric analysis of HeLa cells with DDX5 monoclonal antibody (green) and negative control (red). -
Gene Info — DDX5
Entrez GeneID
1655Gene Name
DDX5
Gene Alias
DKFZp434E109, DKFZp686J01190, G17P1, HLR1, HUMP68, p68
Gene Description
DEAD (Asp-Glu-Ala-Asp) box polypeptide 5
Omim ID
180630Gene Ontology
HyperlinkGene Summary
DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein, which is a RNA-dependent ATPase, and also a proliferation-associated nuclear antigen, specifically reacting with the simian virus 40 tumor antigen. This gene consists of 13 exons, and alternatively spliced transcripts containing several intron sequences have been detected, but no isoforms encoded by these transcripts have been identified. [provided by RefSeq
Other Designations
ATP-dependent RNA helicase DDX5|DEAD box-5|DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 5 (RNA helicase, 68kD)
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Interactome
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Disease
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