Tlr2 monoclonal antibody, clone 7G5F4
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Specification
Product Description
Mouse monoclonal antibody raised against recombinant human Tlr2.
Immunogen
Recombinant protein corresponding to amino acid 628-777 of human Tlr2 from E. coli.
Host
Mouse
Theoretical MW (kDa)
90kDa
Reactivity
Human
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Western Blot (1:500-1:2000)
Immunohistochemistry
Immunocytochemistry (1:200-1:1000)
Flow Cytometry
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.05% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western Blot analysis of (1) HEK293 cells, (2) Tlr2-hIgGFc transfected HEK293 cell lysate.Immunocytochemistry
Immunocytochemical staining of HepG2 cells with Tlr2 monoclonal antibody (green). DRAQ5 fluorescent DNA dye (blue). Actin filaments have been labeled with Alexa Fluor- 555 phalloidin (red).Enzyme-linked Immunoabsorbent Assay
ELISA analysis of Tlr2 monoclonal antibody, clone 7G5F4. -
Gene Info — Tlr2
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Interactome
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