IL1RAPL1 monoclonal antibody, clone 3E3D12
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of Lane 1: A431 cell; Lane 2: SK-Hep-1 cell; Lane 3: HL-7702 cell with IL1RAPL1 monoclonal antibody.
Western Blot (Transfected lysate)
Western Blot analysis of (1) HEK293 cells, (2) IL1RAPL1-hIgGFc transfected HEK293 cell lysate.
Immunocytochemistry
Immunocytochemical staining of HeLa cells with IL1RAPL1 monoclonal antibody (green). DRAQ5 fluorescent DNA dye (blue). Actin filaments have been labeled with Alexa Fluor- 555 phalloidin (red).
Enzyme-linked Immunoabsorbent Assay
ELISA analysis of IL1RAPL1 monoclonal antibody, clone 3E3D12.
Flow Cytometry
Flow cytometric analysis of HeLa cells with IL1RAPL1 monoclonal antibody (green) and negative control (red).
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Specification
Product Description
Mouse monoclonal antibody raised against recombinant human IL1RAPL1.
Immunogen
Recombinant protein corresponding to amino acid 541-694 of human IL1RAPL1 from E. coli.
Host
Mouse
Theoretical MW (kDa)
80kDa
Reactivity
Human
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Western Blot (1:500-1:2000)
Immunohistochemistry
Immunocytochemistry (1:200-1:1000)
Flow Cytometry (1:200-1:400)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.05% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of Lane 1: A431 cell; Lane 2: SK-Hep-1 cell; Lane 3: HL-7702 cell with IL1RAPL1 monoclonal antibody.Western Blot (Transfected lysate)
Western Blot analysis of (1) HEK293 cells, (2) IL1RAPL1-hIgGFc transfected HEK293 cell lysate.Immunocytochemistry
Immunocytochemical staining of HeLa cells with IL1RAPL1 monoclonal antibody (green). DRAQ5 fluorescent DNA dye (blue). Actin filaments have been labeled with Alexa Fluor- 555 phalloidin (red).Enzyme-linked Immunoabsorbent Assay
ELISA analysis of IL1RAPL1 monoclonal antibody, clone 3E3D12.Flow Cytometry
Flow cytometric analysis of HeLa cells with IL1RAPL1 monoclonal antibody (green) and negative control (red). -
Gene Info — IL1RAPL1
Entrez GeneID
11141Gene Name
IL1RAPL1
Gene Alias
IL1R8, IL1RAPL, MRX10, MRX21, MRX34, OPHN4, TIGIRR-2
Gene Description
interleukin 1 receptor accessory protein-like 1
Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of the interleukin 1 receptor family and is similar to the interleukin 1 accessory proteins. It is most closely related to interleukin 1 receptor accessory protein-like 2 (IL1RAPL2). This gene and IL1RAPL2 are located at a region on chromosome X that is associated with X-linked non-syndromic mental retardation. Deletions and mutations in this gene were found in patients with mental retardation. This gene is expressed at a high level in post-natal brain structures involved in the hippocampal memory system, which suggests a specialized role in the physiological processes underlying memory and learning abilities. [provided by RefSeq
Other Designations
OTTHUMP00000023097|X-linked interleukin-1 receptor accessory protein-like 1|interleukin 1 receptor-8|mental retardation, X-linked 10|oligophrenin-4|three immunoglobulin domain-containing IL-1 receptor-related 2
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Interactome
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Disease
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