RAD50 monoclonal antibody, clone 3D2G10
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of Lane 1: C6 cell; Lane 2: HepG2 cell with RAD50 monoclonal antibody.
Western Blot (Transfected lysate)
Western Blot analysis of (1) HEK293 cells, (2) RAD50-hIgGFc transfected HEK293 cell lysate.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of paraffin-embedded esophageal cancer tissues with RAD50 monoclonal antibody.
Enzyme-linked Immunoabsorbent Assay
ELISA analysis of RAD50 monoclonal antibody, clone 3D2G10.
Flow Cytometry
Flow cytometric analysis of HeLa cells with RAD50 monoclonal antibody (green) and negative control (red).
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Specification
Product Description
Mouse monoclonal antibody raised against recombinant human RAD50.
Immunogen
Recombinant protein corresponding to amino acid 228-359 of human RAD50 from E. coli.
Host
Mouse
Theoretical MW (kDa)
154kDa
Reactivity
Human, Rat
Form
Liquid
Isotype
IgG2a
Recommend Usage
ELISA (1:10000)
Western Blot (1:500-1:2000)
Immunohistochemistry (1:200-1:1000)
Immunocytochemistry
Flow Cytometry (1:200-1:400)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.05% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of Lane 1: C6 cell; Lane 2: HepG2 cell with RAD50 monoclonal antibody.Western Blot (Transfected lysate)
Western Blot analysis of (1) HEK293 cells, (2) RAD50-hIgGFc transfected HEK293 cell lysate.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of paraffin-embedded esophageal cancer tissues with RAD50 monoclonal antibody.Enzyme-linked Immunoabsorbent Assay
ELISA analysis of RAD50 monoclonal antibody, clone 3D2G10.Flow Cytometry
Flow cytometric analysis of HeLa cells with RAD50 monoclonal antibody (green) and negative control (red). -
Gene Info — RAD50
Entrez GeneID
10111Gene Name
RAD50
Gene Alias
RAD50-2, hRad50
Gene Description
RAD50 homolog (S. cerevisiae)
Omim ID
604040Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is highly similar to Saccharomyces cerevisiae Rad50, a protein involved in DNA double-strand break repair. This protein forms a complex with MRE11 and NBS1. The protein complex binds to DNA and displays numerous enzymatic activities that are required for nonhomologous joining of DNA ends. This protein, cooperating with its partners, is important for DNA double-strand break repair, cell cycle checkpoint activation, telomere maintenance, and meiotic recombination. Knockout studies of the mouse homolog suggest this gene is essential for cell growth and viability. Two alternatively spliced transcript variants of this gene, which encode distinct proteins, have been reported. [provided by RefSeq
Other Designations
OTTHUMP00000065928|RAD50 homolog
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Interactome
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Pathway
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Disease
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